Summary
Protoplasts of a chloroplast-defective cultivar of Nicotiana tabacum were fused with gamma-irradiated protoplasts of Petunia hybrida. Over 100 photoautotrophic plants were regenerated; of these 94 were tested for Petunia chloroplast traits and all but one had Petunia chloroplasts based on their sensitivity to the fungal toxin, tentoxin. Chloroplast DNA was analysed for 3 of the sensitive plants and was shown to be identical to Petunia chloroplast DNA. Most of the plants (about 70%) appeared to be normal N. tabacum plants, based on morphology and chromosome number. They were fully fertile with normal pollen viability, seed set, and seed viability. The remaining 30% of the plants showed varying degrees of vegetative and reproductive abnormalities.
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Communicated by Yu. Gleba
The techniques of somatic cell genetics have led to many possible nuclear-organellar combinations that may be considered as “cybrids”. In this paper, we use the term to include the combination of nucleus from one species and chloroplast from another species
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Glimelius, K., Bonnett, H.T. Nicotiana cybrids with Petunia chloroplasts. Theoret. Appl. Genetics 72, 794–798 (1986). https://doi.org/10.1007/BF00266547
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DOI: https://doi.org/10.1007/BF00266547