Summary
Diploid homo- and heterokaryotypes of barley translocation lines with only one satellite chromosome pair containing two nucleolus organizer regions (NORs) in opposite arms were found to show repressed nucleolus formation by the transposed NOR as evident from the formation of only micronucleoli. The same was true for auto-tetraploid homokaryotypes and for translocation lines with all NORs tandemly arranged into the same chromosome arm. When NORs were transposed to chromosomes without NOR in the standard karyotype, the normal pattern of nucleolus formation remained unaffected. The modified mode of nucleolus formation after the combination of all NORs in one chromosome pair is interpreted to be due to intrachromosomal nucleolar dominance analogous to interchromosomal nucleolar dominance observed in certain interspecific hybrids.
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Literature
Anastassova-Kristeva, M.: The nucleolar cycle in man. J. Cell Sci. 25, 103–110 (1977)
Anastassova-Kristeva, M., et al: Nucleolus formation in structurally reconstructed barley karyotypes with six satellite chromosomes. Chromosoma 62, 111–117 (1977)
Anastassova-Kristeva, M.; Nicoloff, H.; Rieger, R.; Künzel, G.; Hagberg, A.: Nucleolus organizer activity as affected by chromosome repatterning in barley. Barley Genetics Newsletter, submitted for publication (1979)
Conger, A.D.; Fairchild, L.M.: A quick-freeze method for making smear slides permanent. Stain Technol. 28, 281–283 (1953)
Croce, C.M.; Tacavera, A.; Basilico, C.; Miller, O.J.: Suppression of production of mouse 28 S ribosomal RNA in mouse-human hybrids segregating mouse chromosomes. Proc. nat. Acad. Sci. (Wash.) 74, 694–697 (1977)
Durica, D.S.; Krider, H.M.: Studies on the ribosomal RNA cistrons in interspecific Drosophila hybrids. II. Heterochromatic regions mediating nucleolar dominance. Genetics 89, 37–64 (1978)
Gerstel, D.U.; Burns, J.A.; Burk, L.G.: Cytoplasmic male sterility in Nicotiana, restoration of fertility, and the nucleolus. Genetics 89, 157–169 (1978)
Goodpasture, C.; Bloom, S.E.: Visualization of nucleolus organizer regions in mammalian chromosomes using silver stain. Chromosoma 53, 37–50 (1975)
Heitz, E.: Die Ursache der gesetzmäßigen Zahl, Lage, Form und Größe pflanzlicher Nukleolen. Planta 12, 775–844 (1931)
Honjo, T.; Reeder, R.H.: Preferential transcription of Xenopus laevis ribosomal RNA in interspecies hybrids between X. laevis and X. mulleri. J. Mol. Biol. 80, 217–228 (1973)
Howell, M.W.: Visualization of ribosomal gene activity: Silver stains proteins associated with rRNA transcribed from oocyte chromosomes. Chromosoma 62, 361–367 (1977)
Keep, E.: Nucleolar suppression, its inheritance and association with taxonomy and sex in the genus Ribes. Heredity 26, 443–451 (1971)
Lange, W.; Jochemsen, G.: Karyotypes, nucleoli, and amphiplasty in hybrids between Hordeum vulgare L. and H. bulbosum L. Genetica 46, 217–233 (1976)
Miller, D.A.; Tantrauahi, R.; Dev, V.G.; Miller, O.J.: Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region. Amer. J. hum. Genet. 29, 490–502 (1977)
Navashin, M.S.: Amphiplastie — eine neue karyologische Erscheinung. Proc. int. Conf. Genet. 5, 1148–1152 (1928)
Navashin, M.S.: Chromosome alterations caused by hybridization and their bearing upon certain general genetic problems. Cytologia 5, 169–203 (1934)
Nicoloff, H.; Anastassova-Kristeva, M.; Künzel, G.; Rieger, R.: The behaviour of nucleolus organizers in structurally changed karyotypes of barley. Chromosoma 62, 103–109 (1977)
Nicoloff, H.; Daskaloff, S.: Cytological technique. BAN: Sofia 1966 Schubert, I.; Anastassova-Kristeva, M.; Rieger, R.: Specificity of NOR-staining in Vicia faba. Exptl. Cell Res., submitted for publication (1979)
Varley, J.M.: Patterns of silver staining of human chromosomes. Chromosoma 61, 207–214 (1977)
Wallace, H.; Langridge, W.H.R.: Differential amphiplasty and the control of ribosomal RNA synthesis. Heredity 46, 1–13 (1971)
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Communicated by H.F. Linskens
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Nicoloff, H., Anastassova-Kristeva, M., Rieger, R. et al. ‘Nucleolar dominance’ as observed in barley translocation lines with specifically reconstructed SAT chromosomes. Theoret. Appl. Genetics 55, 247–251 (1979). https://doi.org/10.1007/BF00265358
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DOI: https://doi.org/10.1007/BF00265358