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A method for isolating nonsense suppressors in enterobacteriaceae using an amber mutant of the drug resistance factor R1

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Summary

We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins. We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that contain nonsense suppressors (Sup- strains) by screening penicillin-resistant revertants of strains containing the mutant R factor for the presence of such suppressors. This obviates the need to have known nonsense mutations in chromosomal genes. Theoretically, suppressor-containing derivatives of any bacterial species that can maintain and express R1 can be constructed.

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References

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Authors and Affiliations

  1. A.R.C. Unit of Nitrogen Fixation, University of Sussex, Brighton, UK

    Christina Kennedy & Ian Crowlesmith

  2. Department of Bacteriology, The Medical School, Bristol, UK

    Christina Kennedy & Ian Crowlesmith

Authors
  1. Christina Kennedy
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  2. Ian Crowlesmith
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Communicated by B.A. Bridges

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Kennedy, C., Crowlesmith, I. A method for isolating nonsense suppressors in enterobacteriaceae using an amber mutant of the drug resistance factor R1. Molec. Gen. Genet. 138, 359–362 (1975). https://doi.org/10.1007/BF00264806

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  • DOI: https://doi.org/10.1007/BF00264806

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