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In vitro modification and restriction of phage Φ105C DNA with Bacillus subtilis N cell-free extract

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Summary

The enzymes involved in host-controlled modification and restriction by Bacillus subtilis strain N were detected in cell-free extracts. In the presence of Mg2+, the N-specific endonuclease cleaved unmodified DNA but did not attack ϕ105C· N DNA carrying N-specific modification. The restriction endonuclease required neither SAM nor ATP for its activity. The N-specific modification enzyme was active only in the presence of SAM, indicating that modification in this system is a methylation of DNA.

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Authors and Affiliations

  1. Laboratory of Microbiology, The Institute of Physical and Chemical Research, Saitama, Japan

    Takehiko Shibata & Tadahiko Ando

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  1. Takehiko Shibata
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  2. Tadahiko Ando
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Communicated by W. Arber

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Shibata, T., Ando, T. In vitro modification and restriction of phage Φ105C DNA with Bacillus subtilis N cell-free extract. Molec. Gen. Genet. 138, 269–279 (1975). https://doi.org/10.1007/BF00264796

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  • DOI: https://doi.org/10.1007/BF00264796

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