Summary
In Neurospora crassa limitation for single amino acids normally results in increased formation of enzymes required for amino acid synthesis via ‘general amino acid control’. Glutamine limitation, however, led to comparatively low and delayed derepression of enzyme synthesis. Nitrate reductase activity increased steeply under these conditions confirming that de novo protein synthesis could occur. Derepression levels were unaffected by addition of glutamine-derived metabolites. Only small and delayed increases in mRNA levels occurred for the anabolic enzyme genes arg-12, his-3 and trp-1 under conditions of glutamine limitation in contrast to the immediate and far larger increase found on histidine limitation. The trans-acting regulatory gene of general amino acid control in Neurospora, cpc-1, responded with a significant increase in mRNA level to histidine and to glutamine limitation. The restricted response of the amino acid synthesis genes could imply a post-transcriptional block to the positive regulatory function of cpc-1 under condition of glutamine limitation. The results suggest that the expression of general amino acid control is restricted under conditions of inadequate nitrogen supply.
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References
Barthelmess IB (1982) Mutants affecting amino acid cross-pathway control in Neurospora crassa. Genet Res (Camb) 39:169–185
Bathelmess IB, Kolanus J (1990) The range of amino acids whose limitation activates general control of amino acid synthetic enzymes in Neurospora crassa. Genet Res (Camb) 55:7–12
Bode R, Birnbaum D (1978) Die Biosynthese aromatischer Aminosäuren bei Hansenula henricii: Charkaterisierung der Anthranilat-Syntase. Biochem Physiol Pflanzen 172:245–253
Carsiotis M, Jones RF (1974). Cross-pathway regulation: Tryptophan-mediated control of histidine and arginine biosynthetic enzymes in Neurospora crassa. J Bacteriol 119:889–892
Carsiotis M, Jones RF, Lacy A, Cleary TJ, Frankhauser DB (1970) Histidine-mediated control of tryptophan biosynthetic enzymes in Neurospora crassa. J Bacteriol 104:98–106
Davila G, Sanchez F, Palacios R, Mora J (1978) Genetics and physiology of Neurospora crassa glutamine auxotrophs. J Bacteriol 134:693–698
Davila G, Brom S, Mora Y, Palacios R, Mora J (1983) Genetic and biochemical characterization of glutamine synthetase from Neurospora crassa glutamine auxotrophs and their revertants. J Bacteriol 156:993–1000
Davis RH (1962) A mutant form of ornithine transcarbamylase found in a strain of Neurospora carrying a pyrimidine-proline suppressor gene. Arch Biochem Biophys 97:185–191
Dunn-Coleman NS, Tomsett AB, Garrett RH (1979) Nitrogen metabolite repression of nitrate reductase in Neurospora crassa: Effect of the gln-1a locus. J Bacteriol 139:697–700
Flint HJ (1985) Changes in gene expression elicited by amino acid limitation in Neurospora crassa strains having normal or mutant cross-pathway amino acid control. Mol Gen Genet 200:283–290
Flint HJ, Kemp BF (1981) General control of arginine biosynthetic enzymes in Neurospora crassa. J Gen Microbiol 124:129–140
Flint HJ, McCormick J (1988) Alterations in the pattern of polypeptide synthesis resulting from amino acid limitation in Neurospora crassa. Can J Microbiol 34:1166–1170
Flint HJ, Wilkening J (1986) Cloning of the arg-12 gene of Neurospora crassa and regulation of its transcript via cross-pathway amino acid control. Mol Gen Genet 203:110–116
Garrett RH (1972) The induction of nitrate reductase in Neurospora crassa. Biochem Biophys Acta 264:481–489
Garrett RH, Cove DJ (1976) Formation of NADPH-nitrate reductase activity in vitro from Aspergillus nidulans niaD and cnx mutants. Mol Gen Genet 149:179–186
Hilton JL, Kearney PC, Ames BN (1965) Mode of action of the herbicide 3-amino-1,2,4-triazole (amitrole): inhibition of an enzyme of histidine biosynthesis. Arch Biochem Biophys 112:544–547
Hinnebusch AG (1988) Mechanisms of gene regulation in the general control of amino acid biosynthesis in Saccharomyces cerevisieae. Microbiol Rev 52:248–273
Kemp BF (1982) Cross pathway regulation of amino acid biosynthetic pathways in Neurospora crassa. M Phil Thesis, University of Nottingham
Kolanus J (1990) Regulation der Aminosäurebiosynthesen von Neurospora crassa in Abhängigkeit vom Stickstoffmetabolismus. PhD Thesis, University of Hannover
Legerton TL, Weiss RL (1984) Mobilization of vacuolar arginine in Neurospora crassa. Mechanism and role of glutamine. J Biol Chem 259:8875–8879
Legerton TL, Yanofsky C (1985) Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa. Gene 39:129–140
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Martin RG, Berberich MA, Ames BN, Davis WW, Goldberger RF, Yourno JD (1971) Enzymes and intermediates of histidine biosynthesis in Salmonella typhimurium. Methods Enzymol 17B:3–44
Marzluf GA (1981) Regulation of nitrogen metabolism and gene expression in fungi. Microbiol Rev 45:437–461
Mitchell AP, Magasanik B (1984) Three regulatory systems control production of glutamine synthetase in Saccharomyces cerevisiae. Mol Cell Biol 4:2767–2773
Orbach MJ, Porro EB, Yanofsky C (1986) Cloning and characterization of the gene for β-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol 6:2452–2461
Paluh JL, Orbach MJ, Legerton TL, Yanofsky C (1988) The crosspathway control gene of Neurospora crassa, cpc-1, encodes a protein similar to GCN4 of yeast and the DNA-binding domain of the oncogene v-jun-encoded protein. Proc Natl Acad Sci USA 85:3728–3732
Perkins DD, Radford A, Newmeyer D, Bjorkman M (1982) Chromosomal loci of Neurospora crassa. Microbiol Rev 46:426–570
Premakumar R, Sorger GJ, Gooden D (1978) Stability of messenger RNA for nitrate reductase in Neurospora crassa. Biochim Biophys Acta 519:275–278
Schechtman MG, Yanofsky C (1983) Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli. J Mol Appl Genet 2:83–99
Vogel HJ (1964) Distribution of lysine among fungi: evolutionary implications. Am Nat 98:435–446
Wang L-WC, Marzluf GA (1979) Nitrogen regulation of uricase synthesis in Neurospora crassa. Mol Gen Genet 176:385–392
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Communicated by C. van den Hondel
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Kolanus, J., Michalczyk, J., Flint, H.J. et al. Restricted activation of general amino acid control under conditions of glutamine limitation in Neurospora crassa . Molec. Gen. Genet. 223, 443–448 (1990). https://doi.org/10.1007/BF00264452
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DOI: https://doi.org/10.1007/BF00264452