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Features of squamous and adenocarcinoma in the same cell in a xenografted human transitional cell carcinoma: Evidence of a common histogenesis?

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Summary

Ultrastructural features of squamous differentiation have been found in adenocarcinomatous cells in a xenografted line (UCRU-BL-17) established in nude mice from a primary human bladder transitional cell carcinoma (grade III, stage T4) with a tetraploid DNA component. The line has been characterized by light and electron microscopy, flow cytometry and immunocytochemistry. The initial xenograft showed predominantly adenocarcinomatous differentiation with mucin secretion, whilst the subsequent passages also contained cells showing squamous differentiation. A xenograft subline established from a cell culture of the initial xenograft shows the emergence of a population of cells with near triploid DNA, which are less differentiated, grow more quickly, show decreased expression of carcinoembryonic antigen, and a change in the distribution of staining with peanut lectin from cell surface to cytoplasm. These lines offer an unusual opportunity to study the histogenetic relationships between the histological subtypes of bladder cancer.

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Authors and Affiliations

  1. U.C.R.U. Laboratories, Department of Surgery, University of Sydney, Australia

    P. J. Russell, M. Jelbart & D. Raghavan

  2. Department of Anatomical Pathology, Royal Prince Alfred Hospital, Sydney, Australia

    E. J. Wills

  3. Department of Anatomical Pathology, Royal North Shore Hospital, Sydney, Australia

    J. Philips

  4. Kanematsu Laboratories, Royal Prince Alfred Hospital, Sydney, Australia

    J. Wass

  5. Ludwig Institute for Cancer Research, University of Sydney, Australia

    P. Gregory

  6. Urological Cancer Research Unit Level E9, Royal Prince Alfred Hospital, Camperdown, Australia

    D. Raghavan

Authors
  1. P. J. Russell
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  2. E. J. Wills
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  3. J. Philips
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  4. J. Wass
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  5. M. Jelbart
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  6. P. Gregory
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  7. D. Raghavan
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Additional information

Supported by a grant from the National Health and Medical Research Council of Australia

Supported by grants from N.S.W. State Cancer council and N.S.W. Women's Bowl for Others Club

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Russell, P.J., Wills, E.J., Philips, J. et al. Features of squamous and adenocarcinoma in the same cell in a xenografted human transitional cell carcinoma: Evidence of a common histogenesis?. Urol. Res. 16, 79–84 (1988). https://doi.org/10.1007/BF00261960

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  • DOI: https://doi.org/10.1007/BF00261960

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