Skip to main content
Log in

Detection of antigenic differences and similarities between human transitional cell carcinoma cell lines using rabbit antisera

  • Originals
  • Published:
Urological Research Aims and scope Submit manuscript

Summary

Rabbit antisera to cell lines derived from transitional cell carcinomas were examined by complement dependent cytotoxicity, absorption analysis, and inhibition assays and the staphylococcal protein-A assay. Test cells were from cell lines derived from transitional cell carcinomas (TCC-CL), other tumours, and normal cells. Following several absorptions of the antisera with normal tissues and insolubilized FCS, activity was undet ectable against many carcinoma cell lines but remained against TCC-CL, a lung carcinoma derived line, and many of the non-carcinoma lines. Further absorption of anti-253J (a TCC-CL) antisera with non TCC lines resulted in specific activity against 253J. This selectivity was supported by SpA assay results. Inhibition and absorption experiments using cultured cells and supernatants disclosed a complex quantitative and/or qualitative antigenic relationship between cell lines. Absorption of antisera with tumour and normal tissue specimens and inhibition of cytotoxicity by patients' and controls' sera indicated possible clinical relevance of detected antigens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bean, M. A., Pees, H., Rosen, G., Oettgen, H. F.: Pre-labeling target cells with 3H-proline as a method for studying lymphocyte cytoxicity. National Cancer Institute Monographs 37, 41–48 (1973)

    Google Scholar 

  2. Beidler, J. L., Helson, L., Spengler, B. A.: Morphology and growth, tumorigenicity of human neuroblastoma cells in continuous culture. Cancer Research 33, 2643–2652 (1973)

    Google Scholar 

  3. Bloom, E. T.: Further definition by cytotoxicity tests of cell surface antigens on human sarcomas in culture. Cancer Research 32, 960–967 (1972)

    Google Scholar 

  4. Bloom, E. T.: Antigen(s) on human transitional cell carcinoma detected by a xenogeneic anitserum. Urological Research 5, 141–148 (1977)

    Google Scholar 

  5. Bloom, E. T., Fahey, J. L., Peterson, I. A., Geering, G., Bernhard, M., Trempe, G.: Anti-tumor activity in human serum: antibodies detecting blood group A-like antigen on the surface of tumor cells in culture. International Journal of Cancer 12, 21–31 (1973)

    Google Scholar 

  6. Bloom, E. T., Ossorie, R. C., Brosman, S. A.: Cell-mediated cytotoxicity against human bladder cancer. International Journal of Cancer 14, 326–334 (1974)

    Google Scholar 

  7. Bloom, E. T., Seeger, R. C.: Disease and non-disease-related cell-mediated cytotoxicity in humans. Cancer Research 36, 1361–1366 (1976)

    Google Scholar 

  8. Bubeník, J., Barešová, M., Viklecký, V., Jakoubková, J., Sainerová, H., Donner, J.: Established cell line of urinary bladder carcinoma (T24) containing tumor-specific antigen. International Journal of Cancer 11, 1765–1773 (1973)

    Google Scholar 

  9. Cailleau, R.: The establishment of a cell strain (MA C21) from a mucoid adenocarcinoma of the human lung. Cancer Research 20, 837–841 (1960)

    Google Scholar 

  10. DeVries, J. E., Meyering, M., van Dongen, A., Rümke, P.: The effects of different isolation procedures and the use of target cells from melanoma cell lines and shortterm cultures on the non-specific cytotoxic effects of lymphocytes from healthy donors. International Journal of Cancer 15, 391–400 (1975)

    Google Scholar 

  11. Elliott, A. Y., Bronson, D. L., Stein, N., Fraley, E. E.: In vitro cultivation of epithelial cells dervied from tumors of the human urinary tract. Cancer Research 36, 367–369 (1976)

    Google Scholar 

  12. Elliott, A. Y., Cleveland, P., Cervenka, J., Castro, A. E., Stein, N., Hakala, T. R., Fraley, E. E.: Characterization of a cell line from human transitional cell cancer of the urinary tract. Journal of the National Cancer Institute 53, 1341–1349 (1974)

    Google Scholar 

  13. Fogh, J., Lund, R. O.: Continuous cultivation of epithelial strain (F) from human amniotic membrane. Proceedings of the Society for Experimental Biology and Medicine 94, 532–537 (1957)

    Google Scholar 

  14. Fogh, J., Trempe, G.: “New human tumor cell lines.” In: Fogh, J. (ed.) Human Tumor Cell Lines in Vitro, pp 116–159, Plenum Press, New York (1975)

    Google Scholar 

  15. Gey, G. O., Coffman, W. D., Kubicek, M. T.: Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Research 12, 264–265 (1952)

    Google Scholar 

  16. Hakala, T. R., Lange, P. H., Castro, A. E., Elliott, A., Fraley, E. E.: Cell-mediated cytotoxicity against human transitional cell carcinomas of the genitourinary tract. Cancer 34, 1929–1934 (1974)

    Google Scholar 

  17. McAllister, R. M., Gardner, M. B., Greene, A. E., Bradt, C., Nichols, W. W., Landing, B. H.: Cultivation in vitro of cells derived from a human osteosarcoma. Cancer Research 35, 2684–2688 (1975)

    Google Scholar 

  18. McAllister, R. M., Isaacs, H., Rongey, R., Peer, M., Au, W., Soukup, S. W., Gardner, M. B.: Establishment of a human medulloblastoma line. International Journal of Cancer 20, 206–212 (1977)

    Google Scholar 

  19. Miller, A. B.: The etiology of bladder cancer from the epidemiological viewpoint. Cancer Research 37, 2939–2942 (1977)

    Google Scholar 

  20. O'Toole, C., Nayak, S., Price, Z., Gilbert, W. H., Waisman, J.: A cell line (SCaBER) derived from squamous cell carcinoma of the human urinary bladder. International Journal of Cancer 17, 707–714 (1976)

    Google Scholar 

  21. O'Toole, C., Perlmann, P., Unsgaard, B., Moberger, G., Edsmyr, F.: Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. International Journal of Cancer 10, 77–91 (1972)

    Google Scholar 

  22. O'Toole, C., Perlmann, P., Unsgaard, B., Almgard, L. E., Johansson, B., Moberger, G., Edsmyr, F.: Cellular immunity to human urinary bladder carcinoma. International Journal of Cancer 10, 92–98 (1972)

    Google Scholar 

  23. O'Toole, C., Stejskal, V., Perlmann, P., Karlsson, M.: Lymphoid cells mediating tumor-specific cytotoxicity to carcinoma of the urinary bladder. Journal of Experimental Medicine 139, 457–466 (1974)

    Google Scholar 

  24. Pierce, G. E., DeVald, B.: Microcytotoxicity assays of tumor immunity in patients with clinical status. Cancer Research 35, 3577–3584 (1975)

    Google Scholar 

  25. Rabson, A. S., Rosen, S. W., Tashjian, A. H., Weintraub, B. D.: Production of human chorionic gonadotropia by a cell line derived from a carcinoma of the lung. Journal of the National Cancer Institute 50, 669–674 (1973)

    Google Scholar 

  26. Sussell, W. C., Newman, C., Williamson, D. H.: A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253, 461–462 (1975)

    Google Scholar 

  27. Schlesinger, H. R., Gerson, J. M., Moorhead, H., Maguire, H., Hummeler, K.: Establishment and characterization of human neuroblastoma cell lines. Cancer Research 36, 3094–3100 (1976)

    Google Scholar 

  28. Seeger, R. C., Rayner, S., Banerjee, A., Chung, H., Laug, W. E., Neustein, H., Benedict, W. F.: Morphology, growth, chromosomal pattern, and fibrolytic activity of two new neuroblastoma lines. Cancer Research 37, 1364–1371 (1977)

    Google Scholar 

  29. Sofen, H., O'Toole, C.: Anti-Squamous tumor antibodies in patients with squamous cell carcinoma. Cancer Research 38, 199–203 (1978)

    Google Scholar 

  30. Takasugi, M., Mickey, M. R., Terasaki, P. I.: Reactivity of lymphocytes from normal person on cultures tumor cells. Cancer Research 33, 2898–2902 (1973)

    Google Scholar 

  31. Zeltzer, P. M., Seeger, R. C.: Microassay using radioiodinated protein A from Staphylococcus aureus for antibodies bound to cell surface antigens of adherent tumor cells. Journal of Immunological Methods 17, 163–175 (1977)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bloom, E.T., Brown, D.E. Detection of antigenic differences and similarities between human transitional cell carcinoma cell lines using rabbit antisera. Urol. Res. 8, 5–13 (1980). https://doi.org/10.1007/BF00261381

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00261381

Key words

Navigation