Summary
Ten DNA repair (rad) genes from the fission yeast, Schizosaccharomyces pombe were mapped to the 17 NotI fragments of the three chromosomes. Nine of the genes map to chromosome I, but there is no evidence for significant clustering.
Similar content being viewed by others
References
Anand R (1986) Pulsed field gel electrophoresis: a technique for fractionating large DNA molecules. Trends Genet 2:278–283
Barker DG, White HHM, Johnston LH (1985) The nucleotide sequence of the DNA ligase gene(CDC9) from Saccharomyces cerevisiae: a gene which is cell-cycle regulated and induced in response to DNA damage. Nucleic Acids Res 13:8323–8337
Barker DG, White JHM, Johnston LH (1987) Molecular characterisation of the DNA ligase gene, cdc17 from the fission yeast Schizosaccharomyces pombe. Eur J Biochem 162:659–667
Barnes DE, Johnston LH, Kodama K-I, Tomkinson AE, Lasko DD, Lindahl T (1990) Human DNA ligase I cDNA: Cloning and functional expression in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 87:6679–6683
Beach DB, Piper M, Nurse P (1982) Construction of a Schizosaccharomyces pombe gene bank: a yeast bacterial shuttle vector and its use to isolate genes by complementation. Mol Gen Genet 187:326–329
Fan J-B, Chikashige Y, Smith CL, Niwa O, Yanagida M, Cantor RC (1988) Construction of a NotI restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res 17:2801–2818
Hoeijmakers JHJ, Bootsma D (1990) Molecular genetics of eukaryotic DNA excision repair. Cancer Cells 2:311–320
Lieberman HB, Riley R, Martel M (1989) Isolation and initial characterization of a Schizosaccharomyces pombe mutant exhibiting temperature-dependent radiation sensitivity due to a mutation in a previously unidentified rad locus. Mol Gen Genet 218:554–558
Mohrenweiser HW, Carrano AV, Fertitta A, Perry B, Thompson LH, Tucker JD, Weber CA (1989) Refined mapping of the three DNA repair genes, ERCCI, ERCC2, and XRCCI, on human chromosome 19. Cytogenet Cell Genet 52:11–14
Munz P, Wolf K, Kohli J, Leupold U (1989) Genetics overview. In: Nasim A, Young P, Johnson PF (eds) Molecular Biology of the fission yeast. Academic Press, San Diego, pp 1–30
Phipps J, Nasim A, Miller DR (1985) Recovery, repair and mutagenesis in Schizosaccharomyces pombe. Adv Genet 23:1–72
Reynolds P, Koken MHM, Hoeijmakers JHJ, Prakash S, Prakash L (1990) The rhp6 + gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae. EMBO J 9:1423–1430
Sambrook J, Fritsch EF, Maniatis TE (1989) Molecular Cloning: a laboratory manual (2nd Ed). Cold Spring Harbor Laboratory Press, New York
Southern EM, Anand R, Brown WRA, Fletcher DS (1987) A model for the separation of large DNA molecules by crossed field gel electrophoresis. Nucleic Acids Res 15:5925–5943
Sunnerhagen P, Seaton BL, Nasim A, Subramani S (1990) Cloning and analysis of a gene involved in DNA repair and recombination, the rad1 gene of Schizosaccharomyces pombe. Mol Cell Biol 10:3750–3760
Van Duin M, De Wit J, Odijk H, Westerveld A, Yasui A, Koken MHM, Hoeijmakers JHJ, Bootsma D (1986) Moleclar characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD1O. Cell 44:913–923
Weber CA, Salazar EP, Stewart SA, Thompson LH (1990) ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. EMBO J 9:1437–1448
Weeda G, Van Ham RCA, Vermeulen W, Bootsma D, Van Der Eb AJ, Hoeijmakers JHJ (1990) A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome. Cell 62:777–791
Author information
Authors and Affiliations
Additional information
Communicated by B.J. Kilbey
Rights and permissions
About this article
Cite this article
Broughton, B.C., Barbet, N., Murray, J. et al. Assignment of ten DNA repair genes from Schizosaccharomyces pombe to chromosomal NotI restriction fragments. Molec. Gen. Genet. 228, 470–472 (1991). https://doi.org/10.1007/BF00260641
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00260641