Summary
One part by weight of finely powdered expeller cake from brown mustard seeds (Brassica juncea) was mixed well with 2.5 parts by weight of water and, after addition of 0.2% CelluclastTM (NOVO Industry A/S, Bagsvaerd), the mixture was allowed to stand at 20°C, 35°C or 50°C for 2 h. The enzymatic breakdown was followed using a reductometric method. The mixture was then mixed well with one part by volume of water, the temperature was rapidly raised to 100°C, and the volatile oil was recovered by steam distillation. The yield of the resulting oil from ground samples was approximately 50% higher than that obtained without cellulolytic pretreatment at 35°C. Grinding of the expeller cake enhanced enzymatic digestion. The allyl isothiocyanate content of the oil was estimated by titrimetric and gas-chromatographic methods.
Similar content being viewed by others
References
Food Chemicals Codex III (1981a) Test methods for flavor aromatic chemicals and isolates. Committee of Codex Specifications. Chairman Willard Robinson. National Academy Press, Washington, pp 426–427
Food Chemicals Codex III (1981b) Specifications for flavor aromatic chemicals and isolates. Committee of Codex Specifications. Chairman Willard Robinson. National Academy Press, Washington, p 356
Goering KI (1961) Process of obtaining the proteinaceous feed material from mustard seed, rape seed and similar seeds. U. S. patent no. 2 987 399
Grassmann W, Stadler R, Bender R (1933) Justus Liebigs Annalen der Chemie 502:20–40
Jennings W, Shibamoto T (1980) Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. Academic Press, New York
Klyosov AA, Sinitsyn AP, Rabinowitch ML (1980) The comparative role of exoglucosidase and cellobiase in glucose formation from cellulose. In: Enzyme engineering. Weetall HH, Royer GP (eds) Plenum Press, New York vol 5 pp 153–165
Malmos H (1978) Industrial applications of cellulase (Publication of the American Institute of Chemical Engineers) AICHE Symp Ser 74:93–99
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Palakavayalil T (1971) Method of obtaining flavouring from mustard expeller cake. Indian Patent no. 126 789
Reese ET, Siu RGH, Lewinson HS (1950) The biological degradation of soluble cellulose derivate and its relationship to the mechanism of cellulose hydrolysis. J Bact 59:485–97
Silberschmidt K (1931) Münchener medizinische Wochenschrift. pp 1819–1824
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szakács-Dobozi, M., Halász, A., Kozma-Kovács, E. et al. Enhancement of mustard oil yield by cellulolytic pretreatment. Appl Microbiol Biotechnol 29, 39–43 (1988). https://doi.org/10.1007/BF00258348
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00258348