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The use of replicated progenies in marker-based mapping of QTL's

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Summary

Seven types of progeny are described which can be used in detection of linkage between marker loci and quantitative trait loci (QTL) in a cross between two inbred lines. Three types of progeny: recombinant inbred lines (RI); doubled haploid lines (DH); and S1 lines can be used to detect linked main effects, d. DH and RI lines can be used to detect smaller effects than S1 lines. However, S1 lines can also be used to detect within-population dominance effects, h. The smallest d detectible is in the range of 1/2 to 1/12 the size of the corresponding LSD(0.05) for the quantitative trait, using 100 lines and 6 replicates. The smallest h detectible is 3–4 times this size. Four types of progeny can be used to detect differences in the dominance behavior of alleles within the population relative to an allele in another inbred line (P4: DH lines x P4; RI lines x P4; either F2 x P4 or S1 lines x P4; and progeny generated by crossing (F1 x P3) x P4. Dominance differences in the range of 1 1/4 to 1/6 the size of the corresponding LSD(0.05) are routinely detectible using 100 lines and 6 replicates. Increasing the numbers of progeny evaluated or the number of replicates allows for the detection of relatively smaller linked effects.

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Communicated by A. L. Kahler

Contribution of United AgriSeeds, Inc.

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Cowen, N.M. The use of replicated progenies in marker-based mapping of QTL's. Theoret. Appl. Genetics 75, 857–862 (1988). https://doi.org/10.1007/BF00258045

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  • DOI: https://doi.org/10.1007/BF00258045

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