Summary
Pot experiments were carried out to study the influence of bulk density (D b), soil water tension (pF) and presence of plants (spring wheat) on denitrification in a low-humus Bt-horizon of a udalf. Pots of only 5-cm depth were found to be most suitable for the experiments when using the acetylene inhibition method. Almost homogeneous soil compaction between 1.1 and 1.6g soil cm−3 was achieved by a Proctor tamper. Water tensions were adjusted by means of ceramic plates on which negative pressure was applied. No denitrification was detected in unplanted pots. With planted pots and increasing bulk density denitrification increased more in pots with 14-day-old plants than in pots with 7-day-old plants. With 14-day-old plants N2O emission pot−1 increased steadily from 2 μmol at D b 1.1 to 8 μmol at D b 1.6, when soil moisture was adjusted to pF 1.5, although root growth was impaired by higher bulk density. From an experiment with different bulk densities and water tensions it could be deduced that the air-filled porosity ultimately determined the rate of denitrification. When low water tension was applied for a longer period, water tension had an overriding effect on total denitrification. Denitrification intensity, however, i.e. the amount of N2O g−1 root fresh weight, was highest when low water tension was accompanied by high bulk density. The results suggest that the increase in denitrification intensity at oxygen stress is partly due to higher root exudation.
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References
Benckiser G, Syring KM (1985) Erfassung und Modellierung gasförmiger Stickstoffverluste einer Parabraunerde. Mitt Dtsch Bodenkundl Gesellsch 41:441–450
Benckiser G, Haider K, Sauerbeck D (1986) Field measurements of gaseous nitrogen losses from an alfisol planted with sugar-beets. Z Pflanzenernähr Bodenkd 149:249–261
Brat SS (1972) Influence of roots on denitrification. Plant Soil 36:713–715
Burford JR, Stefanson RC (1973) Measurement of gaseous losses of nitrogen from soils. Soil Biol Biochem 5:133–141
Castillo SR, Dowdy RH, Bradford JM, Larson WE (1982) Effects of applied mechanical stress on plant growth and nutrient uptake. Agron J 74:526–530
Ehlers W (1983) Auswirkung der Bodenbelastung mit schwerem Gerät und der Bodenbearbeitung auf das Bodengefüge und das Pflanzenwachstum. Kali-Briefe 16:499–516
Flühler H, Stolzy LH, Ardakani MS (1976a) A statistical approach to define soil aeration in respect to denitrification. Soil Sci 122:115–123
Flühler H, Ardakani MS, Szuskiewicz TE, Stolzy LH (1976b) Field measured nitrous oxide concentrations, redox potential, oxygen diffusion rates and oxygen partial pressures in relation to denitrification. Soil Sci 122:107–114
Folorunso OA, Rolston DE (1984) Spatial variability of field-measured denitrification gas fluxes. Soil Sci Soc Am J 48:1214–1219
Frede HG, Gebhardt H, Meyer B (1975) Größe, Ursachen und Bedingungen von Boden- und Dünger-N-Verlusten durch Denitrifikation aus dem Ap-Horizont einer Acker-Parabraunerde aus Löß Mitteilung I: Labor-Modell-Versuche mit natürlichen Bodenmonolithen. Göttinger Bodenkundl Ber 34:69–165
Grineva GM (1961) Excretion by plant roots during brief periods of anaerobiosis. Fiziologiya Rastenii 8:686–691 (Transl. in: Am Inst Biol Sci 8:549–552, 1962)
Guenzi WD, Beard WE, Watanabe FS, Olsen SR, Porter LK (1978) Nitrification and denitrification in cattle manure-amended soil. J Environ Qual 7:196–202
Haider K, Mosier AR, Heinemeyer O (1985) Phytotron experiments to evaluate the effect of growing plants on denitrification. Soil Sci Soc Am J 49:636–641
Kézdi A (1969) Handbuch der Bodenmechanik. VEB-Verlag Bauwesen, Berlin
Kowalenko CG, Cameron DR (1978) Nitrogen transformation in soil-plant system in three years of field experiments using tracer and non-tracer methods on an ammonium fixing soil. Can J Soil Sci 58:195–208
Kraffczyk I, Trolldenier G, Beringer H (1984) Soluble root exudates of maize: influence of potassium supply and rhizosphere microorganisms. Soil Biol Biochem 16:315–322
Kroeckel L, Stolp H (1986) Influence of the water regime on denitrification and aerobic respiration in soil. Biol Fertil Soils 2:15–21
Letey J, Jury WA, Hadas Aviva, Valoras N (1980) Gas diffusion as a factor in laboratory incubation studies on denitrification. J Environ Qual 9:223–226
Mohr D (1977) Untersuchungen der Durchwurzelung von Lößböden unterschiedlichen Erosionsgrades mit Hilfe von 32P. Mitt Dtsch Bodenkundl Gesellsch 25:353–356
Moraghan JT, Buresh R (1977) Correction for dissolved nitrous oxide in nitrogen studies. Soil Sci Soc Am J 41:1201–1203
Myrold DD, Tiedje JM (1985) Diffusional constraints on denitrification in soil. Soil Sci Soc Am J 49:651–657
Naguib AI (1956) On the co-existence between nitrate-reducing bacteria and the roots of higher plants. Biol Abstr 33, no 2686
Pilot L, Patrick WH (1972) Nitrate reduction in soils: effect of soil moisture tension. Soil Sci 114:312–316
Rheinbaben W von, Trolldenier G (1983) Denitrification on plants in nutrient solution culture. Z Pflanzenernähr Bodenkd 146:171–179
Rheinbaben W von, Trolldenier G (1984a) Influence of plant growth on denitrification in relation to soil moisture and potassium nutrition. Z Pflanzenernähr Bodenkd 147:730–738
Rheinbaben W von, Trolldenier G (1984b) Denitrifikation im Freilandversuch mit Weidelgras. Landwirtsch Forsch 37:175–180
Sallam A, Jury WA, Letcy J (1984) Measurement of gas diffusion coefficient under relative low air-filled porosity. Soil Sci Soc Am J 48:3–6
Sauerbeck D, Helal MM (1986) Plant root development and photosynthate consumption depending on soil compaction. Berichte XIII. Congress Int Bodenkundl Gesellsch, Hamburg 3:948–949
Smith KA (1980) A model of the extent of anaerobic zones in aggregated soils and its potential application to estimates of denitrification. J Soil Sci 31:263–277
Sommer C (1978) Eine Methode zur kontinuierlichen Wasserversorgung von Vegetationsgefäßen nach dem Bodenwasserpotential. Landbauforsch Völkenrode 28:17–20
Stefanson RC (1972) Soil denitrification in sealed soil-plant systems. I. Effect of plants, soil water content and soil organic matter contenta. Plant Soil 37:113–127
Trolldenier G (1971) Einfluß der Stickstoff- und Kaliumernährung von Weizen sowie der Sauerstoffversorgung der Wurzeln auf Bakterienzahl, Wurzelatmung und Denitrifikation in der Rhizosphäre. Zbl Bakteriol Abt II, 126:130–141
Trolldenier G, Hecht-Buchholz CH (1984) Effect of aeration status of nutrient solution on microorganisms, mucilage and ultrastructure of wheat roots. Plant Soil 80:381–390
Trolldenier G, Wollersheim R (1985) Einfluß der Lagerungsdichte und der Wasserspannung auf die Denitrifikation. Mitt Dtsch Bodenkundl Gesellsch 43:611–615
Woldendorp JW (1962) The quantitative influence of the rhizosphere on denitrification. Plant Soil 17:267–270
Woldendorp JW (1963) The influence of living plants on denitrification. Meded Landbouw Hogeschool Wageningen 63:1–100
Yoshinari T, Knowles R (1976) Acetylene inhibition of nitrous oxide reduction by denitrifying bacteria. Biochem Biophys Res Commun 69:705–710
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Wollersheim, R., Trolldenier, G. & Beringer, H. Effect of bulk density and soil water tension on denitrification in the rhizosphere of spring wheat (Triticum vulgare . Biol Fert Soils 5, 181–187 (1987). https://doi.org/10.1007/BF00256898
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DOI: https://doi.org/10.1007/BF00256898