Skip to main content
Log in

Anticancer drug sensitivity in vitro in the bladder cancer cell line, KK-47 and prophylactic use of carbazilquinone and urokinase in bladder cancer

Urological Research Aims and scope Submit manuscript

Summary

Using colony formation technique and KK-47 cell line established from a human bladder transitional cell carcinoma, the effects of 6 anticancer drugs, thio-TEPA, Bleomycin, mitomycin C, carbazilquinone, Adriamycin and cis-Platinum, were compared. On the results of tests performed to establish the drug concentration required to achive a 50% inhibition of cell survival, carbazilquinone was chosen for the prevention of recurrences of bladder cancer. The two groups studied consisted of 56 patients (previously untreated group) who were rendered free of tumours by surgical intervention and of 19 patients (thio-TEPA failures group) who had experienced a persistent recurrence of tumours after prophylactic thio-TEPA instillations and were presumed free of the recurrence of tumours after the next surgical intervention. The 2 groups were subjected to prophylactic combined intravesical instillation therapy with carbazilquinone and urokinase. In the previously untreated group, 6 of the 56 patients (10. 7%) had a recurrence of tumours, and the recurrence rate after 21 months was 16. 7%, using the actuarial method. In the thio-TEPA failures group, 12 of the 19 patients (63. 2%) had a recurrence of tumours, a rate at 21 months of 76. 1%. A considerable drop in the recurrence rate was obtained by the combined instillation therapy in the previously untreated group. The results in the thio-TEPA failures group suggested the presence of a cross-resistance between both alkylating agents, and of a persistent susceptibility to multifocal lesions. No bone marrow depression was observed but an episode of anaphylactic shock attributable to the use of carbazilquinone occurred in 1 out of a total 75 patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bergkvist, A., Ljungqvist, A., Moberger, G.: Classification on bladder tumours based on the cellular pattern. Preliminary report of a clinical-pathological study of 300 cases with a minimum follow-up of eight years. Acta Chirurgica Scandinavica 130, 371 (1965)

    Google Scholar 

  2. Franksson, C.: Tumours of the urinary bladder. A pathological and clinical study of four hundred and thirty-four cases. Acta Chirurgica Scandinavica 100, 664 (1950)

    Google Scholar 

  3. Heney, N. M., Daly, J., Prout, G. R., Jr., Nieh, P. T., Heney, J. A., Trebeck, N. E.: Biopsy of apparently normal epithelium in patients with bladder carcinoam. Journal of Urology 120, 559 (1978)

    Google Scholar 

  4. Hisazumi, H., Uchibayashi, T., Naito, K., Misaki, T., Miyazaki, K.: The prophylactic use of thio-TEPA and urokinase in transitional cell carcinoma of the bladder: A preliminary report. Journal of Urology 114, 394 (1975)

    Google Scholar 

  5. Hisazumi, H., Saitoh, Y., Katoh, M., Nakajima, K., Kobayashi, T., Tsukahara, K., Uchibayashi, T., Taya, T., Misaki, T., Miyazaki, K., Kuroda, K.: Prophylactic use of anticancer agents and urokinase in bladder cancer. Gan to Cagakuryoho 5, 835 (1978)

    Google Scholar 

  6. Hisazumi, H., Kanokogi, M., Nakajima, K., Kobayashi, T., Tsukahara, K., Naito, K., Kuroda, K., Matsubara, F.: Established cell line of urinary bladder carcinoma (KK-47): growth, heterotransplantation, microscopic structure and chromosome pattern. Japanese Journal of Urology 70, 485 (1979)

    Google Scholar 

  7. Hutchinson, D., Schmid, F. A.: Drug resistance in leukemia. Antibiotics and Chemotherapy 20, 227 (1976)

    Google Scholar 

  8. Melicow, M. M.: Histological study of vesical urothelium intervening between gross neoplasms in total cystectomy. Journal of Urology 68, 261 (1952)

    Google Scholar 

  9. Misaki, T., Hisazumi, H., Uchibayashi, T., Taya, T., Kuroda, K.: The prophylactic use of carboquone and urokinase in bladder carcinoma. Acta Urologica Japonica 26, 683 (1980)

    Google Scholar 

  10. Nakao, H., Arakawa, M., Sindo, H., Masuda, H., Muramatsu, T.: Carboquone, A new anti-cancer agent. Annals Sankyo Research Laboratory 27, 1 (1975)

    Google Scholar 

  11. Nocks, B. N., Nieh, P. T., Prout, G. R., Jr.: A longitudinal study of patients with superficial bladder carcinoma successfully treated with weekly intravesical thio-TEPA. Journal of Urology 122, 27 (1979)

    Google Scholar 

  12. Pavone-Macaluso, M., Caramia, G., Rizzo, F. P.: The use of chemotherapeutic agents in the prophylaxis of papillary bladder tumors. Urologia Internationalis 26, 379 (1971)

    Google Scholar 

  13. Puck, T. T., Marcus, P. I.: Action of X-rays on mammalian cells. Journal of Experimental Medicine 103, 653 (1956)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hisazumi, H., Uchibayashi, T., Katoh, M. et al. Anticancer drug sensitivity in vitro in the bladder cancer cell line, KK-47 and prophylactic use of carbazilquinone and urokinase in bladder cancer. Urol. Res. 9, 231–235 (1981). https://doi.org/10.1007/BF00256892

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00256892

Key words

Navigation