Summary
Isobutene production by Rhodotorula minuta IFO 1102 was studied. It was confirmed that the gas species produced by this yeast was isobutene from the result of analysis with a gas chromatograph mass spectrometer. Oxygen supply was essential to the microbial production of isobutene. The optimum pH was found to be approximately pH 6.0 and optimum temperature 25°–27° C. Isobutene production rate was maximal when l-leucine and l-phenylalanine in the medium were being uptaken by the yeast.
The results from an investigation of the role of l-leucine and l-phenylalanine suggested that l-leucine was the precursor of isobutene and l-phenylalanine the inducer for the enzyme concerned with isobutene production.
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References
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Fujii, T., Ogawa, T. & Fukuda, H. Isobutene production by Rhodotorula minuta . Appl Microbiol Biotechnol 25, 430–433 (1987). https://doi.org/10.1007/BF00253313
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DOI: https://doi.org/10.1007/BF00253313