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Analysis of nucleolar activity in Agropyron elongatum, its amphiploid with Triticum aestivum and the chromosome addition lines

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Summary

The nucleolar organizer activity of the Agropyron elongatum, its amphiploid with hexaploid wheat (Triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. Four Ag-NORs are observed in A. elongatum corresponding to the chromosomes 6E and 7E. In the amphiploid T. aestivum — A. elongatum, eight Ag-NORs are observed which corresponds the wheat chromosomes 1B and 6B and to the elongatum chromosomes 6E and 7E. Thus, there is codominance in the nucleolar organizer activity of the chromosomes of the two species. However, a partial amphiplasty is detected since less than 8 Ag-NORs (7 up to 4) are observed in some metaphase cells; the chromosomes 6E and 7E are occasionally suppressed by wheat chromosomes. This conclusion is confirmed by the behaviour of the addition lines since only in those corresponding to the chromosomes 6E and 7E are the elongatum chromosomes nucleolar active although occasionally they can be suppressed by wheat chromosomes.

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References

  • Appels R, Gerlach WL, Dennis ES, Swift H, Peacock WJ (1980) Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. Chromosoma 78:293–311

    Google Scholar 

  • Cauderon Y (1958) Etudie cytogenetique des Agropyron francais et de leurs hybrids avec les bles. DSc Thesis, l'Université de Paris

  • Cermeño MC, Orellana J, Santos JL, Lacadena JR (1984) Nucleolar competition analysis in wheat, rye and their derivatives by silver-staining procedure. Chromosoma (submitted)

  • Dvořák J (1975) Meiotic pairing between single chromosomes of diploid Agropyron elongatum and decaploid A. elongatum in Triticum aestivum. Can J Genet Cytol 17:329–336

    Google Scholar 

  • Dvořák J (1980) Homoeology between Agropyron elongatum chromosomes and Triticum aestivum chromosomes. Can J Genet Cytol 22:237–259

    Google Scholar 

  • Dvořák J, Knott DR (1974) Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to Triticum aestivum. Can J Genet Cytol 16:399–417

    Google Scholar 

  • Evans LE (1962) Karyotype analysis and chromosome designations for diploid Agropyron elongatum (Host) P.B. Can J Genet Cytol 4:267–271

    Google Scholar 

  • Gerlach WL, Miller TE, Flavell RB (1980) The nucleolus organizers of diploid wheat revealed by in situ hybridization. Theor Appl Genet 58:97–100

    Google Scholar 

  • Giraldez R, Cermeño MC, Orellana J (1979) Comparison of C-banding pattern in the chromosomes of inbred lines and open pollinated varieties of rye, Secale cereale L. Z Pflanzenzücht 83:40–48

    Google Scholar 

  • Heneen WK, Runemark H (1972) Cytology of the Elymus (Agropyron) elongatum complex. Hereditas 70:155–164

    Google Scholar 

  • Hizume M, Sato S, Tanaka A (1980) A highly reproducible method of nucleolus organizing regions staining in plants. Stain Technol 55:87–90

    Google Scholar 

  • Hutchinson J, Miller TE (1982) The nucleolar organizer of tetraploid and hexaploid wheats revealed by in situ hybridisation. Theor Appl Genet 61:285–288

    Google Scholar 

  • Lacadena JR, Cermeño MC, Orellana J, Santos JL (1984) Evidence for wheat-rye nucleolar competition (amphiplasty) in Triticale by silver staining procedure. Theor Appl Genet 67:207–213

    Google Scholar 

  • Matsumura S, Sakamoto S (1955) Karyotypes of diploid Agropyron species. Wheat Inf Serv 2:19

    Google Scholar 

  • Miller TE, Gerlach WK, Flavell RN (1980) Nucleolus organizer variation in wheat and rye revealed by in situ hybridization. Heredity 45:377–382

    Google Scholar 

  • Orellana J, Santos JL, Lacadena JR, Cermeño MC (1983) Nucleolar competition analysis in Aegilops ventricosa and its amphiploids with tetraploid wheats and diploid rye by silver staining procedure. Can J Genet Cytol (submitted)

  • Ramsay G, Dyer AF (1983) Nucleolar organiser suppression in barley x rye hybrids. Brandham PE, Bennett MD (eds) Kew chromosome conference II. George Allen and Unwin, London, pp 361

    Google Scholar 

  • Santos JL, Lacadena JR, Cermeño MC, Orellana J (1983) Nucleolar organizer activity in wheat-barley chromosome addition lines. Heredity (submitted)

  • Sato S, Hizume M, Kawamura S (1980) Relationships between secondary constrictions and nucleolus organizing regions in Allium sativum chromosomes. Protoplasma 105:77–85

    Google Scholar 

  • Schulz-Schaeffer J, Jurasits O (1962) Biosystematic investigation in the genus Agropyron. 1. Cytological studies of species karyotypes. Am J Bot 49:940–953

    Google Scholar 

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Communicated by R. Riley

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Lacadena, J.R., Cermeño, M.C., Orellana, J. et al. Analysis of nucleolar activity in Agropyron elongatum, its amphiploid with Triticum aestivum and the chromosome addition lines. Theoret. Appl. Genetics 68, 75–80 (1984). https://doi.org/10.1007/BF00252316

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  • DOI: https://doi.org/10.1007/BF00252316

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