Summary
The activity of collagenase and certain lysosomal hydrolases (cathepsin B1, cathepsin D, β-glucuronidase and β-N-acetyl glucosaminidase) was studied in serum and tissues of rats with Streptozotocin- or alloxan-induced diabetes. The activity of serum lysosomal enzymes was increased in both groups (p<0.05). Both Streptozotocin- and alloxan-diabetic animals showed significantly higher dermal collagenase activity than those of controls (p<0.01), but the liver and spleen showed similar activities; there was a significant decrease in the renal collagenase activity of streptozotocin-diabetic rats (p<0.05). Comparison of the alloxan- or streptozotocin-treated groups with control animals showed an increase in lysosomal enzymes (cathepsin B1, cathepsin D, β-glucuronidase and β-N-acetyl glucosaminidase in skin, liver and spleen) (p<0.05) but β-N-acetyl glucosaminidase was unchanged in the spleen of streptozotocin-diabetic rats. There was no difference in renal cathepsin B1 and D in control versus alloxandiabetic rats, but there was an increase in β-glucuronidase and β-N-acetyl glucosaminidase (p<0.05). The streptozotocindiabetic animals showed decreased activities of renal lysosomal enzymes (p<0.05), but similar activity of cathepsin D to the control animals.
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Mohanam, S., Bose, S.M. Influence of streptozotocin- and alloxan-induced diabetes in the rat on collagenase and certain lysosomal enzymes in relation to the degradation of connective tissue proteins. Diabetologia 25, 66–70 (1983). https://doi.org/10.1007/BF00251900
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DOI: https://doi.org/10.1007/BF00251900