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Protein content of peptidoglycan of liquid-grown cells differs from that of surface-grown, gliding Cytophaga johnsonae

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Abstract

The peptidoglycan sacculi of surface-grown Cytophaga johnsonae had associated with them a large amoutn of protein (the major species is 50 kDa) whereas sacculi from liquid-grown cells had little or no attached protein. The 50 kDa protein was localized in the outer membrane of liquid-grown cells. A portion of this membrane-derived 50 kDa protein was attached to the peptidoglycan only when the cells made contact with the substratum. Protein synthesis did not appear to be required for attachment as the process was not inhibited by chloramphenicol. Association of the 50 kDa protein with the peptidoglycan in response to cell contact with the substratum is suggested.

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Pitta, T., Godchaux, W. & Leadbetter, E.R. Protein content of peptidoglycan of liquid-grown cells differs from that of surface-grown, gliding Cytophaga johnsonae . Arch. Microbiol. 160, 214–221 (1993). https://doi.org/10.1007/BF00249127

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  • DOI: https://doi.org/10.1007/BF00249127

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