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The dependence of the cytoplasmic pH in aerobic and anaerobic cells of the green algae Chlorella fusca and Chlorella vulgaris on the pH of the medium as determined by 31P in vivo NMR spectroscopy

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Abstract

The pH in the cytoplasm of aerobic and anaerobic cells of the green algae Chlorella fusca and Chlorella vulgaris was determined in dependence on the pH of the external medium, which was varied between pH 3 and pH 10. In aerobic cells of both species the cytoplasmic pH is maintained at a value above 7.2 even at an external pH of 3 and below 7.8 at an external pH of 10. In anaerobic cells the cytoplasmic pH shows linear dependence on external pH in the range of pH 6 to 9 (cytoplasmic pH 6.9 to 7.2), while below an external pH of 6 cytoplasmic pH is maintained at about 6.5.

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Abbreviations

CCCP:

Carbonylcyanide-m-chlorophenyl-hydrazone

EDTA:

Ethylendiaminetetraacetic acid

MES:

2-(N-Morpholino)-ethanesulfonic acid

MOPSO:

3-(N-Morpholino)-2-hydroxy-propanesulfonic acid

NMR:

Nuclear Magnetic Resonance

pH cyt:

cytoplasmic pH

pH ex:

external pH

PIPES:

Piperazine-N,N′-bis(2-ethanesulfonic acid)

PPi :

Pyrophosphate

PP1, PP2, PP3 :

1st, 2nd, 3rd phosphate group of polyphosphates

PP4 :

core phosphate groups of polyphosphates

TRIS:

Tris-hydroxymethyl-aminomethane

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Author notes

  1. A. C. Küsel

    Present address: Central spectroscopy, German Cancer Research Center, Im Neuenheimer Feld 280, D-6900, Heidelberg, FRG

Authors and Affiliations

  1. FB Biologie, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    A. C. Küsel & L. H. Grimme

  2. Chemie, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    D. Leibfritz

  3. FB Physik, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    J. Sianoudis & A. Mayer

Authors
  1. A. C. Küsel
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  2. J. Sianoudis
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  3. D. Leibfritz
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  4. L. H. Grimme
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  5. A. Mayer
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Küsel, A.C., Sianoudis, J., Leibfritz, D. et al. The dependence of the cytoplasmic pH in aerobic and anaerobic cells of the green algae Chlorella fusca and Chlorella vulgaris on the pH of the medium as determined by 31P in vivo NMR spectroscopy. Arch. Microbiol. 153, 254–258 (1990). https://doi.org/10.1007/BF00249077

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  • DOI: https://doi.org/10.1007/BF00249077

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