Abstract
A new acidophilic, mineral sulphide oreoxidizing bacterium was isolated from a uranium mine near Salamanca, Spain. Cells were rod-shaped, motile and gram-negative. They were aerobes, could grow on pyrite and use sulphur or thiosulphate as sole energy source, suggesting this new isolate belongs to the genus Thiobacillus. It could grow neither with glucose nor with yeast extract as sole substrates. It could not grow on ferrous sulphate as the only energy source, although it grew in the same medium supplemented with glucose, yeast extract or thiosulphate. It was a mesophilic and extremely acidophilic Thiobacillus, with an optimal pH of 1.5 2. The G+C content of the DNA was 58%. The new isolate could grow in cultures on pyrite where electrophoretic pattern was clearly different from those of other thiobacilli, such as T. ferrooxidans.
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Abbreviations
- G+C:
-
Guanine + Cytosine
References
Acevedo F, Gentina JC (1989) Process engineering aspects of the bioleaching of copper ores. Bioproc Eng 4: 223–229
Balashova VV, Vedenina IY, Markosyan GE, Zavarzin GA (1974) The autotrophic growth of Leptospirillum ferrooxidans. Mikrobiology 43: 581–585
Berry VK, Murr LE (1980) Morphological and ultrastructural study of the cell envelope of thermophilic and acidophilic microorganisms as compared to Thiobacillus ferrooxidans. Biotechnol Bioeng 22: 2543–2555
Bryant RD, McGroarty KM, Costerton JW, Laishley EJ (1983) Isolation and characterization of a new acidophilic Thiobacillus specie (T. albertis). Can J Microbiol 29: 1159–1170
Colmer AR, Hinkle ME (1947) The role of microorganisms in acid mine drainage: a preliminary report. Science 106: 253–256
Guay R, Silver M (1975) Thiobacillus acidophilus sp. nov., isolation and some physiological characteristics. Can J Microbiol 21: 281–288
Huber H, Stetter KO (1989) Thiobacillus prosperus sp. nov., represents a new group of halotolerant metal-mobilizing bacteria isolated from a marine geothermal field. Arch Microbiol 151: 479–485
Huber M, Stetter KO (1990) Thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium. Appl Environ Microbiol 56: 315–322
Huber G, Huber H, Stetter KO (1986) Isolation and characterization of a new metal-mobilizing bacteria. Biotechnol Bioeng Symp 16: 239–251
Ingledew JW (1986) Ferrous iron oxidation by Thiobacillus ferrooxidans. Biotechnol Bioeng Symp 16: 23–33
Jackson PJH (1985) Bacterial taxonomy based on electrophoretic whole-cell protein pattern. Soc Appl Bacteriol Tech Ser 20: 115–129
Kelly PD, Harrison AP (1989) Genus Thiobacillus. In: Staley JT, Bryant MP, Pfennig N, Holt JG (eds) Bergey's manual of systematic bacteriology, vol 3. Williams & Wilkins, Baltimore, pp 1842–1858
Lowry OH, Rosebrough NJ, Farr AL, Randall J (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118
Muir MK, Andersen TN (1977) Determination of ferrous iron in copper process metallurgical solutions by the o-phenanthroline colorimetric method. Metall Trans [B] 8: 517–518
Norris PR (1983) Iron and mineral oxidation with Leptospirillum-like bacteria. In: Rossi G, Torma AE (eds) Recent progress in biohydrometallurgy. Associazione Mineraria Sarda, Iglesias, pp 83–96
Norris PR (1989) Factors affecting bacterial mineral oxidation: the example of carbon dioxide in the context of bacterial diversity. In: Salley J, McCready RGL, Wichlacz PL (eds) Biohydrometallurgy. Proceedings of the International Symposium. Can Met, pp 3–14
Norris PR (1990) Acidophilic bacteria and their activity in mineral sulfide oxidation. In: Ehrlich HL, Brierley CL (eds) Microbial mineral recovery. McGraw-Hill, New York, pp 3–27
Pichuantes S, Garrido J, Leighton V, Rodriguez M (1986) Studies on native strains of Thiobacillus ferrooxidans. II. Comparative fine structure. Biotechnol Appl Biochem 8: 284–291
Pronk JT, Meesters PJW, Dijken JP van, Bos P, Kuenen JG (1990) Heterotrophic growth of T. acidophilus in batch and chemostat cultures. Arch Microbiol 153: 392–298
Torma AE (1988) Leaching of metals. In: Rehm HJ, Reed G (eds) Biotechnology, vol 6b. VCH, Weinheim, pp 368–399
Yates JR, Holmes D (1987) Two families of repeated DNA sequences in Thiobacillus ferrooxidans. J Bacteriol 169: 1861–1870
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de Silóniz, M.I., Lorenzo, P., Murúa, M. et al. Characterization of a new metal-mobilizing Thiobacillus isolate. Arch. Microbiol. 159, 237–243 (1993). https://doi.org/10.1007/BF00248478
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DOI: https://doi.org/10.1007/BF00248478