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Magnetic resonance imaging reflects cartilage proteoglycan degradation in the rabbit knee

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Abstract

Cartilage degeneration in osteoarthritis is initiated by a loss of proteoglycan. Intra-articular injection of papain causes a reversible loss of proteoglycan in rabbit knees. Rabbits were scanned with magnetic resonance imaging (MRI), using a 1.5T Signa superconducting magnet with 3 inch surface coil. Spin echo sequences were performed in the coronal and sagittal planes at 0, 24, 48, and 72 h after intra-articular injection of papain to obtain T1, proton density, and T2-weighted images. Cartilage proteoglycan content was measured biochemically and histochemically. Reduced articular cartilage thickness in the MR images of papain-treated knees corresponded to changes in cartilage proteoglycan content.

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Authors and Affiliations

  1. Pharmaceutical Division, Research Department, Ciba-Geigy Corporation, Summit, New Jersey, USA

    Pradip Kumar Paul M.B.B.S., Elizabeth O'Byrne Ph.D., Vincent Blancuzzi M.S., Douglas Wilson B.A., Diane Gunson B.V.Sc., Ph.D. & Frank L. Douglas Ph.D., M.D.

  2. Laurie Imaging Center, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey, USA

    Jin Zhao Wang Ph.D. & Reuben S. Mezrich M.D., Ph.D.

Authors
  1. Pradip Kumar Paul M.B.B.S.
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  2. Elizabeth O'Byrne Ph.D.
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  3. Vincent Blancuzzi M.S.
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  4. Douglas Wilson B.A.
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  5. Diane Gunson B.V.Sc., Ph.D.
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  6. Frank L. Douglas Ph.D., M.D.
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  7. Jin Zhao Wang Ph.D.
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  8. Reuben S. Mezrich M.D., Ph.D.
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Paul, P.K., O'Byrne, E., Blancuzzi, V. et al. Magnetic resonance imaging reflects cartilage proteoglycan degradation in the rabbit knee. Skeletal Radiol. 20, 31–36 (1991). https://doi.org/10.1007/BF00243718

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