Abstract
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1.
The dependence of dark-adapted fly (Calliphora vicina) photoreceptors on oxygen was investigated by measuring the electroretinogram (ERG), the receptor potential, and the redox states of the mitochondrial cytochromes. The redox states were determined via reflection microspectrophotometry on white-eyed fly mutants.
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2.
The light sensitivity of the photoreceptors at oxygen fractions above ≈ 2% is identical to that in the normal (air) environment, as judged from both ERG and receptor potential. Light sensitivity is rather abruptly and strongly reduced at oxygen fractions lower than ≈ 1% and vanishes at anoxia (0%).
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3.
The redox state of the mitochondrial cytochromes also changes around oxygen fractions of 1–2%, but the dependence on oxygen fraction is less abrupt than that of ERG and receptor potential.
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4.
The mitochondrial activity of dark-adapted fly photoreceptors appears to be well-buffered, to warrant ample supply of metabolic energy for unimpaired photoreceptor function down to extremely low oxygen levels.
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References
Autrum H (1958) Electrophysiological analysis of the visual systems in insects. Exp Cell Res, Suppl 5: 426–439
Autrum H, Hoffmann C (1960) Diphasic and monophasic responses in the compound eye of Calliphora. J Insect Physiol 4: 122–127
Boschek CB (1971) On the fine structure of the peripheral retina and lamina ganglionaris of the fly, Musca domestica. Z Zellforsch 118: 369–409
Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol 17: 65–134
Coombe PE (1986) The large monopolar cells L1 and L2 are responsible for ERG transients in Drosophila. J Comp Physiol A 159: 655–665
Dimitracos SA, Tsacopoulos M (1985) The recovery from a transient inhibition of the oxidative metabolism of the photoreceptors of the drone (Apis mellifera). J Exp Biol 119: 165–181
Hamdorf K, Hochstrate P, Höglund G, Burbach B, Wiegand U (1988) Light activation of the sodium pump in blowfly photoreceptors. J Comp Physiol A 162: 285–300
Hardie RC (1985) Functional organization of the fly brain. In: Ottoson D (ed) Progress in sensory physiology, vol 5. Springer, Berlin Heidelberg New York, pp 1–79
Harold FM (1986) The vital force: a study in bioenergetics. Freeman, New York
Hoffmann C (1960) Belichtungspotentiale der Insekten und Sauerstoffdruck. Verh Dtsch Zool Ges 53: 220–225
Jansonius NM (1990) Properties of the sodium pump in the blowfly photoreceptor cell. J Comp Physiol A 167: 461–467
Järemo Jonson A-C, Nilsson D-E (1994) Effects of energy deprivation on the fly pupil mechanism: evidence for a rigor state. J Comp Physiol A 174: 701–706
Langer H (1975) Properties and functions of screening pigments in insect eyes. In: Snyder AW, Menzel R (eds) Photoreceptor optics. Springer, Berlin Heidelberg New York, pp 429–455
Mojet MH (1992) Phototransduction and light-induced mitochondrial activation in blowfly compound eyes. Thesis Groningen
Smits RP, Stavenga DG (1994) Light-induced changes in retinal oxygen concentration of the blowfly measured in vivo. In: Elsner N, Breer H (eds) Sensory transduction. Proceedings of the 22nd Göttingen neurobiology conference 1994, vol I. Thieme, Stuttgart New York, 37
Stavenga DG (1989) Pigments in compound eyes. In: Stavenga DG, Hardie RC (eds) Facets of vision. Springer, Berlin Heidelberg New York London Paris Tokyo, pp 152–172
Stavenga DG, Schwemer J (1984) Visual pigments of invertebrates. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum, New York, pp 11–61
Tinbergen J, Stavenga DG (1986) Photoreceptor redox state monitored in vivo by transmission and fluorescence microspectrophotometry in blowfly compound eyes. Vision Res 26: 239–243
Trujillo-Cenóz O (1972) The structural organization of the compound eye in insects. In: Fuortes MGF (ed) Handbook of sensory physiology, vol VII/2. Springer, Berlin Heidelberg New York, pp 5–61
Trumpower BL, Gennis RB (1994) Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: the enzymology of coupling electron transfer reactions to transmembrane proton translocation. Ann Rev Biochem 63: 675–716
Tsacopoulos M, Poitry S, Borsellino A (1981) Diffusion and consumption of oxygen in the superfused retina of the drone (Apis mellifera) in darkness. J Gen Physiol 77: 601–628
Tzagaloff A (1982) Mitochondria. Plenum, New York
Wong F, Lantz RC, Mauro A (1979) The role of calcium ions in the suppression of the photoresponse during anoxia and application of metabolic inhibitors. Vision Res 19: 251–254
Zimmerman RP (1978) Field potential analysis and the physiology of second-order neurons in the visual system of the fly. J Comp Physiol 126: 297–316
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Smits, R.P., Jansonius, N.M. & Stavenga, D.G. Dependence of receptor potential and redox state of mitochondrial cytochromes on oxygen fraction measured in the blowfly eye in vivo. J Comp Physiol A 177, 105–110 (1995). https://doi.org/10.1007/BF00243402
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DOI: https://doi.org/10.1007/BF00243402