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Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum

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Summary

Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in ‘heavy’, junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr ∼ 400 000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.

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Authors and Affiliations

  1. Department of Biochemistry, University of North Carolina, 27599, Chapel Hill, NC, USA

    Gerhard Meissner, Eric Rousseau, F. Anthony Lai, Qi-Yi Liu & Kristin A. Anderson

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  1. Gerhard Meissner
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  2. Eric Rousseau
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  3. F. Anthony Lai
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  4. Qi-Yi Liu
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  5. Kristin A. Anderson
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Meissner, G., Rousseau, E., Lai, F.A. et al. Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum. Mol Cell Biochem 82, 59–65 (1988). https://doi.org/10.1007/BF00242517

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  • DOI: https://doi.org/10.1007/BF00242517

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