Abstract
Patterns of proteins excreted during pollen germination, of germinating pollen and of the stylar and placental fluids excreted by cells along the pistil pathway of Gasteria verrucosa were analyzed by electrophoresis. The medium from germinating pollen contains several pollen exudate proteins as well as glycoproteins from the sticky pollen wall coating. No protein could be detected in the stigmatic exudate. The stylar fluid shows a protein pattern different from that of the placental fluid. The placental fluid contains some glycoproteins. After pollination, three pollen proteins begin to appear in the stylar fluid. Two of these pollen proteins remain present in the placental fluid. Some placental fluid proteins and glycoproteins are modified after pollination. The difference in protein patterns demonstrates the heterogeneity of proteins in the pollen tube pathway and suggests that proteins excreted by the pollen tube interact with other proteins in the pistil pathway, especially those in the placental fluid.
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Willemse, M.T.M., Vletter, A. Appearance and interaction of pollen and pistil pathway proteins in Gasteria verrucosa (Mill.) H. Duval. Sexual Plant Reprod 8, 161–167 (1995). https://doi.org/10.1007/BF00242261
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DOI: https://doi.org/10.1007/BF00242261