Summary
Rat cortical synaptosomes were incubated in a saline medium. On the addition of pentobarbital, chloralhydrate, ether and protoveratrine a redistribution of synaptic vesicles was revealed by electron microscopy. The anaesthetics decreased the number of synaptic vesicles attached to the presynaptic membrane while protoveratrine increased them. It is assumed that these agents affect the mechanism of vesicle reformation from the synaptosomal membrane and/or vesicle migration towards the synaptic cleft.
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References
Barker, I.L., Gainer, H.: Pentobarbital: selective depression of excitatory postsynaptic potentials. Science 182, 720–722 (1973)
Berl, S., Nicklas, W.J.: Contractile proteins in relation to transmitter release. In: Metabolic compartmentation and neurotransmission (eds. S. Berl, D.D. Clarke and D. Schneider), pp. 247–271. New York, London: Plenum Press 1975
Blaustein, M.P., Goldring, J.M.: Membrane potentials in pinched-off presynaptic nerve terminals monitored with a fluorescent probe: evidence that synaptosomes have potassium diffusion potentials. J Physiol (Lond) 247, 589–615 (1975)
Bradford, H.F.: Membrane potentials and metabolic performance in mammalian synaptosomes. In: Defects in cellular organelles and membranes in relation to mental retardation (ed. P.F. Benson), pp. 1–15. London: Churchill 1971
Bradford, H.F., Bennett, G.W., Thomas, A.J.: Depolarizing stimuli and the release of physiologically active amino acids from suspensions of mammalian synaptosomes. J Neurochem 21, 495–505 (1973)
Ceccarelli, F.G., Hurlbut, W.P., Mauro, A.: Turnover of transmitter and synaptic vesicles at the neuromuscular junction. J Cell Biol 57, 499–524 (1973)
Edwards, C., Larrabee, M.G.: Effects of anaesthetics on metabolism and on transmission in sympathetic ganglia of rats: measurement of glucose in microgram quantities using glucose oxidase. J Physiol (Lond) 130, 456–466 (1955)
Escueta, A.V., Appel, S.H.: Biochemical studies of synaptosomes in vitro. II. Potassium transport. Biochemistry 8, 725–733 (1969)
Hajós, F.: An improved method for the preparation of synaptosomal fractions in high purity. Brain Res 93, 485–489 (1975)
Hajós, F., Csillag, A.: Structural changes in vitro of isolated nerve endings. I. Effect of cations. Brain Res 112, 207–213 (1976)
Jones, D.G., Bradford, H.F.: Observations on the morphology of mammalian synaptosomes following their incubation and electrical stimulation. Brain Res 28, 491–499 (1971)
Jones, S.F., Kwanbunbumpen, S.: The effects of nerve stimulation and hemicholinium on synaptic vesicles at the mammalian neuromuscular junction. J Physiol (Lond) 207, 31–50 (1970)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity in electron microscopy. J Cell Biol 27, 137A (1964)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265–275 (1951)
Loyning, Y., Oshima, T., Yokota, T.: Site of action of thyamytal sodium on the monosynaptic spinal reflex pathway in cats. J Neurophysiol 27, 408–428 (1964)
Marchbanks, R.M.: Osmotically sensitive potassium and sodium compartments of synaptosomes. Biochem J 104, 148–157 (1967)
Nicoll, R.A.: The effects of anesthetics on synaptic excitation and inhibition in the olfactory bulb. J Physiol (Lond) 223, 803–814 (1972)
Nicoll, R.A.: Presynaptic action of barbiturates in the frog spinal cord. Proc Nat Acad Sci (Wash) 72, 1460–1463 (1975a)
Nicoll, R.A.: Pentobarbital: action on frog motoneurons. Brain Res 96, 119–123 (1975b)
Pysh, J.J., Wiley, R.G.: Synaptic vesicle depletion and recovery in cat sympathetic ganglia electrically stimulated in vivo. Evidence for transmitter release by exocytosis. J Cell Biol 60, 365–374 (1974)
Quilliam, J.P., Tamarind, D.L.: Local vesicle populations in rat superior cervical ganglia and the vesicle hypothesis. J Neurocytol 2, 59–75 (1973)
Streit, P., Akert, K., Sandri, C., Livingston, R.B., Moor, H.: Dynamic ultrastructure of presynaptic membranes at nerve terminals in the spinal cord of rats. Anesthetized and unanesthetized preparations compared. Brain Res 48, 11–26 (1972)
Uchizono, K.: Characteristics of excitatory and inhibitory synapses in the central nervous system of the cat. Nature 207, 642–643 (1965)
Weakly, J.N.: Effect of barbiturates on quantal synaptic transmission in spinal motoneurons. J Physiol (Lond) 204, 63–77 (1969)
Widmark, E.: Die theoretischen Grundlagen und die praktische Verwendbarkeit der gerichtlichen-medizinischen Alkoholbestimmung. Wien: Urban und Schwarzenberg 1932
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Hajós, F., Csillag, A. & Kálmán, M. The effect of pentobarbital, chloralhydrate, ether and protoveratrine on the distribution of synaptic vesicles in rat cortical synaptosomes. Exp Brain Res 33, 91–99 (1978). https://doi.org/10.1007/BF00238797
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DOI: https://doi.org/10.1007/BF00238797