Summary
The activity of choline acetyltransferase (ChAT) in the cerebellar lobules of the rat was determined after bilateral destruction of either the superior, middle, or inferior cerebellar peduncles. A significant, 40–60% decrease in ChAT activity occurred in all subdivisions of the cerebellum (anterior lobe, posterior lobe hemisphere, posterior lobe vermis/nodulus and flocculus/paraflocculus) following bilateral inferior peduncle lesions. In contrast, bilateral lesions of the superior or middle peduncles did not result in significant reductions of enzyme activity. These findings indicate that the cholinergic afferents to the cerebellum are contained predominantly in the inferior peduncle, from which they emerge to innervate all of the gross subdivisions of the cerebellum.
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References
Altman J, Das GD (1970) Postnatal changes in the concentration and distribution of cholinesterase in the cerebellar cortex of rats. Exp Neurol 28: 11–34
Armstrong DM, Saper CB, Levey AI, Wahrer BH, Terry RD (1982) Immunocytochemical localization of choline acetyltransferase in the rat brain. Soc Neurosci Abstr 8: 662
Austin L, Phillis JW (1965) The distribution of cerebellar cholinesterases in several species. J Neurochem 12: 709–727
Brodal A (1967) Anatomical studies of cerebellar fibre connections with special reference to problems of functional localization. In: Fox CA, Snider RS (eds) The Cerebellum. Prog Brain Res 25: 135–173
Crepel F, Dhanjal SS (1982) Cholinergic mechanisms and neurotransmission in the cerebellum of the rat. An in vitro study. Brain Res 244: 59–68
Csillik B, Joo F, Kása P (1963) Cholinesterase activity of archicerebellar mossy fiber apparatus. J Histochem Cytochem 11: 113–114
Fonnum F (1972) Application of microchemical analysis and subcellular fractionation techniques to the study of neurotransmitters in discrete areas of mammalian brain. Adv Biochem Psychopharmacol 6: 75–88
Fonnum F (1975) A rapid radiochemical method for the determination of choline acetyltransferase. J Neurochem 24: 407–409
Friede RL, Fleming LM (1964) A comparison of cholinesterase distribution in the cerebellum of several species. J Neurochem 11: 1–7
Kan KSK, Chao LP, Eng LF (1978) Immunohistochemical localization of choline acetyltransferase in rabbit spinal cord and cerebellum. Brain Res 146: 221–229
Kása P, Silver A (1969) The correlation between choline acetyltransferase and acetylcholinesterase activity in different areas of the cerebellum of rat and guinea pig. J Neurochem 16: 389–396
Kása P, Bánsághy K, Rakonczay Z, Guly K (1982) Postnatal development of the acetylcholine system in different parts of the rat cerebellum. J Neurochem 24: 1726–1732
Kimura H, McGeer PL, Peng JH, McGeer EG (1981) The central cholinergic system studied by choline acetyltransferase immunohistochemistry in the cat. J Comp Neurol 200: 151–201
Kimoto Y, Satoh K, Sakumoto T, Tohyama M, Shimizu N (1978) Afferent fiber connections from the lower brain stem to the rat cerebellum by the horseradish peroxidase method combined with MAO staining, with special reference to noradrenergic neurons. J Hirnforsch 19: 85–100
Krnjević K (1974) Chemical nature of synaptic transmission in vertebrates. Physiol Rev 54: 418–540
Nieoullon A, Dusticier N (1981) Decrease in choline acetyltransferase acitvity in the red nucleus of the cat after cerebellar lesion. Neuroscience 6: 1633–1641
Pellegrino LJ, Cushman AJ (1969) A stereotaxic atlas of the rat brain. Appleton-Century-Crofts, New York
Phillis JW (1965) Cholinergic mechanisms in the cerebellum. Br Med Bull 21: 26–29
Phillis JW (1968) Acetylcholinesterase in the feline cerebellum. J Neurochem 15: 691–698
Ross CD, Smith JT, Godfrey DA (1981) Distributions of choline acetyltransferase and acetylcholinesterase activities in regions and layers of rat cerebellum. Soc Neurosci Abstr 7: 76
Rotter A, Birdsall NJM, Field PM, Raisman G (1979) Muscarinic receptors in the central nervous system of the rat. II. Distribution of binding of [3H]propylbenzilylcholine mustard in the midbrain and hindbrain. Brain Res Rev 1: 167–183
Silver A (1967) Cholinesterases of the central nervous system with special reference to the cerebellum. Int Rev Neurobiol 10: 57–109
Shute CCD, Lewis PR (1965) Cholinesterase-containing pathways of the hindbrain: Afferent cerebellar and centrifugal cochlear fibres. Nature 205: 242–246
Sperti L, Sperti S (1959) Effects of chronic lesions of the peduncles on cerebellum cholinesterase activity in the albino rat. Experientia 15: 441–442
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Supported by NINCDS post doctoral fellowship No. 1F32NS06399-02
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Asin, K.E., Satoh, K. & Fibiger, H.C. Regional cerebellar choline acetyltransferase activity following peduncular lesions. Exp Brain Res 53, 370–373 (1984). https://doi.org/10.1007/BF00238167
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DOI: https://doi.org/10.1007/BF00238167