Summary
3H-GABA was injected into restricted regions of visual areas 1 and 2 (cortical areas 17 and 18) on the lateral surface of the occipital lobe in monkeys. The injected tissue was processed for Golgi impregnation and gold toning. Sections containing Golgi-impregnated neurons were re-embedded, sectioned at 1 μm, and prepared for autoradiography to reveal neurones that had selectively accumulated 3H-GABA.
Golgi-impregnated pyramidal, spiny stellate and aspiny nonpyramidal neurons were examined for 3H-GABA accumulation. Out of 47 aspiny nonpyramidal neurons 16 were labelled by 3H-GABA. The other cell types did not accumulate the amino acid. Twelve of the labelled neurons were drawn. Eight were bitufted neurons with their dendrites oriented predominantly radially, three were small multipolar neurons, and one could be reconstructed only partially. One neuron had a locally arborizing axon in layer III.
Two bitufted, Golgi-impregnated neurons in layer II and upper III of area 18 were labelled from GABA injection radially beneath in layer VI, providing evidence for earlier suggestions that in the monkey's visual cortex the cells in the upper layers which project radially and accumulate 3H-GABA are aspiny non-pyramidal cells. The results indicate the existence of different types of putative GABA-ergic interneurons.
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During part of this project P. Somogyi was supported by the Wellcome Trust at the Department of Pharmacology, Oxford University. We are grateful to the Hungarian Academy of Sciences, the International Cultural Institute of Budapest, the Wellcome Trust, the Royal Society, the E.P. Abraham Cephalbsporin Trust, and the Medical Research Council for financial support
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Somogyi, P., Kisvárday, Z.F., Freund, T.F. et al. Characterization by Golgi impregnation of neurons that accumulate 3H-GABA in the visual cortex of monkey. Exp Brain Res 53, 295–303 (1984). https://doi.org/10.1007/BF00238158
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DOI: https://doi.org/10.1007/BF00238158