Summary
A number of investigators have recently used osmotic minipumps to continuously deliver the neurotoxin 6-hydroxydopamine (6-OHDA) to kitten cerebral cortex for periods up to 7 days. Because this compound is known to be particularly labile, we studied the stability of 6-OHDA stored under conditions similar to those found in an osmotic minipump. In 0.4% ascorbic acid, 4 mM 6-OHDA-HBr was found to be stable for at least one week as determined by (1) assay of the drug by high performance liquid chromatography and electrochemical detection and (2) test of the drug's ability to deplete mouse heart norepinephrine.
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References
Anton AH, Sayre DF (1962) A study of the factors affecting the aluminum oxidetrihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther 138: 360–375
Bear MF, Daniels JD (1983) The plastic response to monocular deprivation persists in kitten visual cortex after chronic depletion of norepinephrine. J Neurosci 3: 407–416
Daw NW, Rader RK, Robertson TW, Videen TO (1983a) Do short term and long term depletion of noradrenaline have different effects on visual deprivation in kitten visual cortex? Neurosci Abst 9: 1217
Daw NW, Robertson TW, Rader RK, Videen TO (1983b) Effects of lesions of the dorsal noradrenergic bundle on plasticity in the visual cortex. Invest Ophthal (Suppl.) 24(3): 140
Haycock DA, Patrick RL (1984) Dihydroxyphenylalanine production in rat brain striatal synaptosomes: stimulation by a calcium chelator. J Neurochem 42: 911–917
Heikkila R, Cohen G (1972) Further studies on the generation of hydrogen peroxide by 6-hydroxydopamine. Mol Pharmacol 8: 241–248
Jonsson G (1980) Chemical neurotoxins as denervation tools in neurobiology. Ann Rev Neurosci 3: 169–187
Kasamatsu T, Pettigrew JD (1976) Depletion of brain catecholamines: Failure of ocular dominance shift after monocular occlusion in kittens. Science 194: 206–209
Kasamatsu T, Pettigrew JD, Ary M (1979) Restoration of visual cortical plasticity by local microperfusion of norepinephrine. J Comp Neurol 185: 163–182
Kasamatsu T, Itakura, T, Jonssson G (1981) Intracortical spread of exogenous catecholamines: effective concentration for modifying cortical plasticity. J Pharmacol Exp Ther 217: 841–850
Kostrzewa RM, Jacobowitz DM (1974) Pharmacological actions of 6-hydroxydopamine. Pharmacol Rev 26: 199–288
Paradiso MA, Bear MF, Daniels JD (1983) Effects of intracortical infusion of 6-hydroxydopamine on the response of kitten visual cortex ot monocular deprivation. Exp Brain Res 51: 413–422
Petitjean F, Laguzzi, F, Sordet F, Jouvet M, Pujol JF (1972) Effects de l'injection intraventriculaire de 6-hydroxydopamine I. Sur les monoamines cerebrales du chat. Brain Res 48: 281–293
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We acknowledge the support of NIMH grant MH31706 to Dr. R.L. Patrick, NIH grant NS13031 to Dr. F.F. Ebner and ONR contract N0014-82-K-0136 to Dr. L.N. Cooper. We are grateful for the technical assistance of Robert J. Clinton, Jr.
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Haycock, D.A., Bear, M.F. The stability of 6-hydroxydopamine under minipump conditions. Exp Brain Res 56, 183–186 (1984). https://doi.org/10.1007/BF00237455
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DOI: https://doi.org/10.1007/BF00237455