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Micropropagation of Madhuca longifolia (Koenig) MacBride var. latifolia Roxb.

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Abstract

Bud break and multiple shoots were induced in apical and axillary meristems derived from 10-d old seedlings of Madhuca longifolia var. latifolia on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l N6-benzyladenine (BA) singly or in combinatiobn with 1-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA). Excised shoots were rooted on half-strength MS with IBA (1.0 mg/l) after 18d of culture. Regenerated plantlets were acclimatized and successfully transferred to soil.

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Abbreviations

BA:

N6 benzyladenine

KN:

kinetin

ADS:

adenine sulphate

IBA:

indole-3-butyric acid

IAA:

indole3-acetic acid

NAA:

1-naphthaleneacetic acid

MS:

Murashige and Skoog (1962) medium

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Regional Plant Resource Centre, 751015, Bhubaneswar, India

    G. R. Rout & P. Das

Authors
  1. G. R. Rout
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  2. P. Das
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Communicated by G. C. Phillips

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Rout, G.R., Das, P. Micropropagation of Madhuca longifolia (Koenig) MacBride var. latifolia Roxb.. Plant Cell Reports 12, 513–516 (1993). https://doi.org/10.1007/BF00236098

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  • DOI: https://doi.org/10.1007/BF00236098

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