Changes in the pattern of expression of alkaline phosphatase in the mouse uterus and placenta during gestation

Abstract

The development of the rodent chorio-allantoic placenta is a complicated process that results in the formation of a transport system capable of sustaining embryonic and fetal growth and development. Intimately linked to this process is alkaline phosphatase (AP), a cell-surface glycoprotein that possibly functions as a transport protein. In the present study, we have mapped the location of AP-expressing cells in the mouse utero-placental unit during the development of the chorio-allantoic placenta by use of enzyme histochemistry and in situ hybridization histochemistry. We found that at implantation the expression of the tissue non-specific AP (TNAP) gene is located exclusively in the decidua and that most of this decidual expression ceases as the placenta starts to form. One exception is a mesometrially located marginal zone of the decidua, which continues to express the TNAP gene until day 12 and the active protein until at least day 16. Trophoblasts of the chorion already express AP before the time of fusion with the ectoplacental cone, after which AP is expressed by trophoblasts of the resulting ectoplacental plate. AP expression in the mature chorio-allantoic placenta is localized in the placental labyrinth and spongy zones. In the latter zone, expression ceases on about day 14. Giant trophoblasts start to express AP on about day 10, with some cells still positive for AP at day 16. The yolk sac does not express AP at any developmental stage. The results show that AP expression during placental development is neither restricted to cells known to be involved in transport, nor expressed in all cells thought to be involved in this transport. This may indicate that AP is not merely a transport protein but has additional functions.