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Plant regeneration from protoplasts of wild rice (Oryza rufipogon Griff.)

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Abstract

Embryogenic callus initiated from basal segments of micropropagated shoots of Oryza rufipogon were used to initiate cell suspension cultures. After approximately 3 months these cultures were capable of yielding large numbers of protoplasts which underwent sustained division in agarose-solidified medium at a frequency comparable to that observed with Japonica rice protoplasts in previous studies. O. rufipogon plants were reproducibly regenerated from the protoplast-derived callus and are currently being grown to maturity. This is the first report of plant regeneration from protoplasts of a wild species of Oryza.

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Abbreviations

BAP:

6-benzyalamino purine

2,4-D:

2,4-dichlorophenoxyacetic acid

MES:

2[N-morpholino] ethanesulphonic acid

NAA:

α-naphthalene acetic acid

PAR:

photosynthetically active radiation

SCV:

settled-cell volume

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Author notes

  1. Abdul Baset

    Present address: Plant Breeding Division, Bangladesh Rice Research Institute, Bangladesh, India

  2. Robert P. Finch

    Present address: Department of Plant Science, Scottish Agricultural College, Ayr KA6 5HW, Auchincruive, Scotland

Authors and Affiliations

  1. Plant Genetic Manipulation Group. Department of Botany, University of Nottingham, NG7 2RD, Nottingham, UK

    Abdul Baset, Robert P. Finch & Edward C. Cocking

Authors
  1. Abdul Baset
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  2. Robert P. Finch
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  3. Edward C. Cocking
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Additional information

Communicated by I. Protrykus

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Baset, A., Finch, R.P. & Cocking, E.C. Plant regeneration from protoplasts of wild rice (Oryza rufipogon Griff.). Plant Cell Reports 10, 200–203 (1991). https://doi.org/10.1007/BF00234295

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  • DOI: https://doi.org/10.1007/BF00234295

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