Summary
Electron microscopic examination of Purkinje Cells was performed in sections from the cerebellum of three albino rats aged 4 1/2 month, intoxicated with diphenylhydantoin for 51 days. Three untreated albino rats served as controls.
There were no difference between the substructure of the Purkinje cells from the two groups of animals.
It was concluded that diphenylhydantoin in toxic but sublethal doses does not change the substructure of the Purkinje cells.
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References
Dam, M.: Number of Purkinje cells in patients with grand mal epilepsy treated with diphenylhydantoin. Epilepsia 11, 313–320 (1970a).
—: The number of Purkinje's cells after diphenylhydantoin intoxication in pigs. Arch. Neurol. (Chic.) 22, 64–67 (1970b).
—: The number of Purkinje cells after diphenylhydantoin intoxication in monkeys. Epilepsia 11, 199–206 (1970c).
—, Nielsen, M.H.: Purkinje's cell density after diphenylhydantoin intoxication in rats. Arch. Neurol. (Chic.) 23, 555–558 (1970).
del Cerro, M.P., Snider, R.S.: Studies on Dilantin® intoxication. I. Ultrastructural analogies with Lipoidoses. Neurology (Minneap.) 17, 452–466 (1967).
Fraska, J.M., Parks, V.R.: A routine technique for double staining ultrathin sections using Uranyl and lead salts. J. Cell Biol. 25, 157–161 (1965).
Haberland, C.: Cerebellar degeneration with clinical manifestation in chronic epileptic patients. Psychiat. et Neurol. (Basel) 143, 29–44 (1962).
Herndon, R.M.: The fine structure of the Purkinje cell. J. Cell Biol. 18, 167–180 (1963).
Hofmann, W.W.: Cerebellar lesions after parenteral Dilantin administration. Neurology (Minneap.) 8, 210–214 (1958).
Högelmeier, H., Wenzel, U.: Zerebellarer Dauerschaden durch vorübergehende Hydantoin-überdosierung. Dtsch. med. Wschr. 94, 1330–1332 (1969).
Karlsson, U., Schultz, R.L.: Fixation of the central nervous system for electron microscopy by aldehyde perfusion. III. Structural changes after exsanguination and delayed perfusion. J. Ultrastruct. Res. 14, 47–63 (1966).
Kokenge, R., Kutt, H., McDowell, F.: Neurological sequelae following Dilantin overdose in a patient and experimental animals. Neurology (Minneap.) 15, 823–829 (1965).
Latta, H., Osvaldo, L., Jackson, J.D., Cook, M.L.: Changes in renal cortical tubules during autolysis. Electron microscopic observations. Lab. Invest. 14, 635–657 (1965).
Livingston, S.: The diagnosis and treatment of convulsive disorders in children, Springfield, Ill.:Ch. C. Thomas 1954.
—: Drug therapy for childhood epilepsy. J. chron. Dis. 6, 46–80 (1957).
Maunsbach, A.B.: The influence of different fixatives and fixation methods on the ultrastructure of rat kidney proximal tubule cells. I. Comparison of different perfusion fixation methods and of glutaraldehyde, formaldehyde and osmium tetroxide fixatives. J. Ultrastruct. Res. 15, 242–282 (1966).
Reynolds, E.S.: The use of lead citrate at a high pH as electron opaque stain in the electron microscope. J. Cell Biol. 17, 208–212 (1963).
Roger, J., Soulayrol, R.: A propos des accidents neurologiques du traitement de l'épilepsie par les Hydantoines. Rev. neurol. B. Aires 100, 783–785 (1959).
Snider, R.S., del Cerro, M.P.: Membranous cytoplasmic spirals in Dilantin intoxication. Nature (Lond.) 212, 536–537 (1966).
—: Drug-induced dendritic sprouts on Purkinje cells in adult cerebellum. Exp. Neurol. 17, 466–480 (1967).
Utterback, R.A.: Parenchymatous cerebellar degeneration complicating Diphenylhydantoin (Dilantin®) therapy. Arch. Neurol. Psychiat. (Chic.) 80, 180–181 (1958).
Viukari, M.: The effects of Diphenylhydantoin on the central nervous system. Duodecim (Helsinki) 78, 136–140 (1962).
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This work was supported by grant from the Danish Epilepsy Association and the Danish Medical Research Council.
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Nielsen, M.H., Dam, M. & Klinken, L. The ultrastructure of Purkinje cells in diphenylhydantoin intoxicated rats. Exp Brain Res 12, 447–456 (1971). https://doi.org/10.1007/BF00234242
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DOI: https://doi.org/10.1007/BF00234242