Summary
Wheat proembryos were excised 7 days after anthesis and germinated to normal fertile plants with an efficiency of 90%. These embryos had clearly differentiated shoot apical meristems and represent an interesting model system for studies of meristem biology. A combination of two media with different osmolarities allowed the continued development of the embryos before induction of germination. Normal germination occurred only after the first leaf primordium had already covered the shoot apical meristem.
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References
Dieterich K (1924) Flora 117: 379–417
Haagen-Smit AJ, Siu R, Wilson G (1945) Science 101: 234
Heimann R, Müller M (1991) J Electron Microscopy Tech. 18: 440–449
Iglesias VA, Gisel A, Leduc N, Zhang S, Potrykus I, Sautter C (1993a) J Cellular Biochem 17B: 29
Iglesias VA, Gisel A, Bilang R, Leduc N, Potrykus I, Sautter C (1993b) Planta 192: 84–91
Kent N, Brink RA (1947) Science 106: 547–548
Kent-Ziebur N, Brink RA, Graf LH, Stahlmann MA (1950) Am J Bot 37: 144–148
Kent-Ziebur N, Brink RA (1951) Am J Bot 38: 253–256
King RW (1976) Planta 132: 43–51
Liu C, Xu Z, Chua N-H (1993) Plant J 3: 291–300
Merry J (1941) Bulletin of the Torrey Botanical Club 68: 585–598
Monnier M (1976) Rev Cyt Biol Veg 39: 1–120
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Norstog K (1965) Amer J Bot 52: 538–546
Norstog K (1972) Phytomorphology 22: 134–139
Norstog K, Klein R (1972) Can J Bot 50: 1887–1894
Pope MN (1949) J Agric Res 78: 295–309
Radley M (1979) J Exp Bot 30: 381–389
Raghavan V (1986) Embryogenesis in Angiocperms. Cambridge University Press
Rappaport J (1954) Bot Review 20: 201–225
Triplett BA, Quatrano RS (1982) Dev Biol 91: 491–496
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Communicated by P. J. King
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Iglesias, V.A., Gisel, A., Potrykus, I. et al. In vitro germination of wheat proembryos to fertile plants. Plant Cell Reports 13, 377–380 (1994). https://doi.org/10.1007/BF00234141
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DOI: https://doi.org/10.1007/BF00234141