Skip to main content
SpringerLink
Log in

Adventitious bud regeneration from leaf expiants of the shrubby ornamental honeysuckle, Lonicera nitida Wils. cv. ‘Maigrün’: effects of thidiazuron and 2,3,5-triiodobenzoic acid

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Different combinations of auxins and cytokinins were employed to assess the regeneration capacity from in vitro leaf explants of Lonicera nitida Wils. cv ‘Maïgrün’. A high frequency of rhizogenesis was noticed, with 2.3 μM thidiazuron plus 2.9 μM indole-3-acetic acid as the only hormonal combination to support caulogenic responses. Increasing thidiazuron concentration and/or suppressing auxin did not improve caulogenesis. Combining thidiazuron with 2,3,5-triiodobenzoic acid produced a dramatic increase in the percentage of caulogenic explants. A maximum of 74% of adventitious bud forming explants was obtained with 2.3 μM thidiazuron plus 20 μM 2,3,5-triiodobenzoic acid. Buds were often in a rosette form and were vitreous, so that shoot elongation was difficult to obtain. The effect of the duration of the 2,3,5-triiodobenzoic acid treatment on shoot elongation was investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

GA3:

gibberellic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

2 IP:

2-isopentenyladenine

MS:

Murashige and Skoog

NAA:

α-naphthylacetic acid

TDZ:

N-phenyl-N′-1,2,3-thidiazol-5-ylurea (thidiazuron)

TIBA:

2,3,5-triiodobenzoic acid

Z:

zeatin

References

  • Belaizi M, Paul H, Sangwan RS, Sangwan-Norreel BS (1991) Plant Cell Reports 9: 471–474.

    Article  CAS  PubMed  Google Scholar 

  • Bonnett HT, Torrey JG (1965) Plant Physiol. 40: 1228–1236.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Boonnour K, Wainwright H, Higks RGT (1988) Acta Horticulturae 226: 183–189.

    Article  Google Scholar 

  • Cassels AC (1979) Physiol. Plant. 46: 159–164.

    Article  Google Scholar 

  • Chevreau E, Skirvin RM, Abu-Qaoud HA, Korban SS, Sullivan JG (1989) Plant Cell Reports 7: 688–691.

    CAS  PubMed  Google Scholar 

  • Christianson ML, Warnick DA (1983) Dev. Biol. 95: 288–293.

    Article  CAS  PubMed  Google Scholar 

  • Depta H, Eisele KH, Hertel R (1983) Plant Sc. Lett. 31: 181–192.

    Article  CAS  Google Scholar 

  • Depta H, Rubery PH (1984) J. Plant Physiol. 115: 371–387.

    Article  CAS  PubMed  Google Scholar 

  • Dufour M (1988) Thèse de Doctorat Université des Sciences et Techniques du Languedoc, France.

  • Fasolo F, Zimmerman RH, Fordham I (1989) Plant Cell Tissue and Organ Culture 16: 75–87.

    Article  CAS  Google Scholar 

  • Lainé H, Puska R, Koivunen K, Tormala T (1990) XXIII International Horticultural Congress, Firenze (Italy) August 27-September 1. Poster 1065.

  • McCown BH (1989) In: Bajaj Y P S (ed) “Biotechnology in Agriculture and Forestry 5. Trees II” Springer-Verlag Berlin Heidelberg New-York Tokyo (pp: 324–341).

    Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogaï Y, Okamoto T (1982) Phytochemistry 21: 1509–1511.

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Newman D (1939) Biometrika 31: 20–30.

    Article  Google Scholar 

  • Ochatt SJ (1991) Plant Cell Tissue and Organ Culture 25: 161–167.

    Google Scholar 

  • Rubery P H (1987) In: Davies PJ (ed) “Plant hormones and their role in plant growth and development”. Martinus Nijhoff/Dr W. Junk Publ, Dordrecht (pp 341–362).

    Chapter  Google Scholar 

  • Skoog F, Miller C O (1957) Symp. Soc. Exp. Biol. 11: 118–140.

    CAS  PubMed  Google Scholar 

  • Suzuki T, Inagawa Y, Harada T, Yakuwa T (1986) Memoirs Fac. of Agric., Hokkaido Univ. Japan 15(1):104–110.

    Google Scholar 

  • Thompson KS, Hertel R, Muller S, Tavares JE (1973) Planta 109: 337–352.

    Article  Google Scholar 

  • Van Aartrijck J, Blom-Barnhoorn GJ (1984) J. Plant Physiol. 116: 409–416.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. André Briant Jeunes Plants SA “La Bouvinerie”, BP 15, 49180, St. Barthélémy d'Anjou, France

    Jocelyne Cambecèdes

  2. INRA, Station d'Amélioration des Espèces Fruitières et Ornementales, Rue Georges Morel, 49070, Beaucouzé, France

    Michel Duron & Luc Decourtye

Authors
  1. Jocelyne Cambecèdes
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michel Duron
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Luc Decourtye
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. Potrykus

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cambecèdes, J., Duron, M. & Decourtye, L. Adventitious bud regeneration from leaf expiants of the shrubby ornamental honeysuckle, Lonicera nitida Wils. cv. ‘Maigrün’: effects of thidiazuron and 2,3,5-triiodobenzoic acid. Plant Cell Reports 10, 471–474 (1991). https://doi.org/10.1007/BF00233817

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00233817

Keywords

Search

Navigation