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Production of virus resistant and insect tolerant transgenic tobacco plants

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Abstract

The cucumber mosaic virus coat protein (CMV-CP) gene and a modified Bacillus thuringiensis δ-endotoxin (Bt toxin) gene were cloned into plant expression vector pE3. Tobacco (Nicotiana tabacum cv. G28) leaf discs were transformed with Agrobacterium tumefaciens A12 carrying recombinant pE14. Transgenic r0 and R1 tobacco plants expressing CMV-CP and Bt toxin genes were protected from CMV infection as well as feeding damage of Manduca Sexta (tobacco hornworm) larvae. These results demonstrate that it is feasible to breed new cultivars with multiple resistances via genetic engineering.

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Abbreviations

CMV:

cucumber mosaic virus

Bt toxin:

Bacillus thuringiensis δ-endotoxin

CaMV:

cauliflower mosaic virus

NOS:

nopaline synthase

Kan:

Kanamycin

Spe:

spectinomycin

Carb:

Carbenicillin

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Authors and Affiliations

  1. The National Laboratory of Protein Engineering and Plant genetic Engineering, Peking University, 100871, Beijing, China

    Xiao-you Liang, Yu-xian Zhu & Zhang-liang Chen

  2. Laboratory of Molecular Genetics, College of Biology, Beijing Agricultural University, 100094, Beijing, China

    Xiao-you Liang & Jing-jiu Mi

Authors
  1. Xiao-you Liang
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  2. Yu-xian Zhu
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  3. Jing-jiu Mi
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  4. Zhang-liang Chen
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Communicated by R. N. Beachy

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Liang, Xy., Zhu, Yx., Mi, Jj. et al. Production of virus resistant and insect tolerant transgenic tobacco plants. Plant Cell Reports 14, 141–144 (1994). https://doi.org/10.1007/BF00233778

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  • DOI: https://doi.org/10.1007/BF00233778

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