Skip to main content
SpringerLink
Log in

Glutamate receptor binding in insects and mammals

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

High affinity stereospecific binding sites for L-glutamate have been reported in several regions of mammalian brain. The binding sites in the hippocampus and cerebellum have been studied more extensively than binding in other brain regions. The hippocampal and cerebellar binding sites show similar properties with respect to their pharmacology and their independence of Na+. There is evidence, particularly good in the case of hippocampus, of mechanisms that may regulate the availability of the binding sites in both brain areas. Some progress has been made with the isolation of the hippocampal binding site but the protein has not been extensively characterised.

In the case of insect muscle, high-affinity stereospecific binding of L-glutamate to whole membrane preparations, to detergent-solubilised membranes and to isolated proteolipids has been reported. Much greater variability in the binding characteristics is seen than is the case with the mammalian brain preparations. Preliminary experiments suggest that at least four distinct binding sites may be present on insect muscle.

The complete characterisation of glutamate binding sites is at present precluded by a lack of potent agonists and antagonists. However, recent advances in the pharmacological classification of receptor sites for the excitatory amino acids in mammalian brain could provide sufficient information to permit the identification of the binding sites as synaptic receptors. Invertebrate toxins whose site of action is the insect neuromuscular junction may well prove to be useful tools with which to isolate and characterise the synaptic receptor proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hayashi, T., 1953. Keio J. Med. 3: 183–192.

    Google Scholar 

  2. Krnjevic, K. & Phillis, J. W., 1963. J. Physiol. (London) 165: 274–304.

    Google Scholar 

  3. Curtis, D. R., 1979. Glutamic acid: Advances in Biochemistry and Physiology (Filer, L. J., Garatini, S., Kare, M. R., Reynolds, W. A. & Wurtman, R. J., eds.) pp. 163–175, Raven Press, New York.

  4. Usherwood, P. N. R., 1978. Adv. Comp. Physiol. Biochem. 7: 227–309.

    PubMed  Google Scholar 

  5. Divac, I., Fonnum, F. & Storm-Mathisen, J., 1977. Nature, 266: 377–378.

    PubMed  Google Scholar 

  6. McGeer, E. G. & McGeer, P. L., 1976. Nature, 263: 517–519.

    PubMed  Google Scholar 

  7. Hudson, D. B., Valcana, T., Bean, G. & Timiras, P. S., 1976. Neurochem. Res. 1: 83–92.

    Google Scholar 

  8. Storm-Mathisen, J., 1977. Prog. Neurobiol. 8: 119–181.

    PubMed  Google Scholar 

  9. Kerkut, G. A., Shapira, A. & Walker, R. J., 1965. Comp. Biochem. Physiol. 16: 37–48.

    PubMed  Google Scholar 

  10. Usherwood, P. N. R. & Machili, P., 1966. Nature 210: 633–636.

    Google Scholar 

  11. Usherwood, P. N. R. & Machili, P., 1968. J. Exp. Biol. 49: 341–361.

    Google Scholar 

  12. Beranek, R. & Miller, P. L., 1968. J. Exp. Biol. 49: 83–93.

    Google Scholar 

  13. Anwyl, R. & Usherwood, P. N. R., 1976. J. Physiol. (London) 254: 46–47.

    Google Scholar 

  14. Anderson, C. R., Cull-Candy, S. G. & Miledi, R., 1976. Nature, 261: 151–153.

    PubMed  Google Scholar 

  15. Usherwood, P. N. R. (ed.), 1975. Insect Muscle, Academic Press, London.

    Google Scholar 

  16. Lunt, G. G., 1975. Insect Biochemistry and Function (Candy, D. J. & Kilby, B. A. eds.) pp. 285–306, Chapman and Hall, London.

  17. Filbin, M., Lunt, G. G. & Donnellan, J. F., 1980. Receptors for Neurotransmitters Hormones and Pheromones in Insects (Sattelle, D. B., Hall, L. M. & Hildebrand, J. G., eds.) pp. 153–160, Elsevier/North Holland, Amsterdam.

  18. Heidmann, T., & Changeux, J.-P., 1978. Annu. Rev. Biochem. 47: 317–357.

    PubMed  Google Scholar 

  19. Barnard, E. A., Dolly, J. O., Lang, B., Lo, M. & Shorr, R. G., 1979. Adv. Cytopharmacol. 3: 409–435.

    PubMed  Google Scholar 

  20. Yamamura, H. I., Enna, S. J. & Kuhar, M. J. (eds.), 1978. Neurotransmitter Receptor Binding, Raven Press, New York.

    Google Scholar 

  21. Cuatrecasas, P. & Hollenburg, M. D., 1975. Biochem. Biophys. Res. Commun. 62: 31–41.

    PubMed  Google Scholar 

  22. Bennett, J. P., 1978. Neurotransmitter Receptor Binding (Yamamura, H. L., Enna, S. J. & Kuhar, M. J., eds.) pp. 57–90, Raven Press, New York.

  23. Roberts, P. J., 1974. Nature, 252: 399–401.

    PubMed  Google Scholar 

  24. Michaelis, E. K., Michaelis, M. L. & Boyarsky, L. L., 1974. Biochim. Biophys. Acta, 367: 338–348.

    PubMed  Google Scholar 

  25. Bogdanski, D. F., Tassari, A. H. & Brodie, B. B., 1970. Biochim. Biophys. Acta, 219: 189–199.

    PubMed  Google Scholar 

  26. Logan, W. J. & Snyder, S. H., 1971. Nature, 234: 297–299.

    Google Scholar 

  27. Balcar, V. J. & Johnson, G. A. R., 1972. J. Neurochem. 19: 2657–2666.

    PubMed  Google Scholar 

  28. Foster, A. C. & Roberts, P. J., 1978. J. Neurochem. 31: 1467–1477.

    PubMed  Google Scholar 

  29. Simon, J. R., Contrera, J. F. & Kuhar, M. J., 1976. J. Neurochem. 26: 141–147.

    PubMed  Google Scholar 

  30. Sharif, N. A. & Roberts, P. J., 1980. J. Neurochem. 34: 779–784.

    PubMed  Google Scholar 

  31. Roberts, P. J., 1981. Glutamate: Transmitter in the central Nervous System (Roberts, P. J., Storm-Mathisen, J. & Johnston, G. A. R., eds.) pp. 35–54. John Wiley and Sons, London.

  32. Michaelis, E. K., Grubbs, R. D., Belieu, R. M. & Michaelis, M. L., 1981. Proc. Nat. Acad. Sci. U.S.A.

  33. Sharif, N. A. & Roberts, P. J., 1980. Brain Res. 194: 594–597.

    PubMed  Google Scholar 

  34. Baudry, M. & Lynch, G., 1979. Eur. J. Pharmacol. 57: 283–285.

    PubMed  Google Scholar 

  35. Baudry, M. & Lynch, G., 1981. J. Neurochem. 26: 811–820.

    Google Scholar 

  36. Baudry, M. & Lynch, G., 1980. Proc. Natl. Acad. Sci. USA 77: 2298–2304.

    PubMed  Google Scholar 

  37. Baudry, M., Oliver, M., Creager, R., Weirasko, A. & Lynch, G., 1980. Life Sci. 27: 325–330.

    PubMed  Google Scholar 

  38. Baudry, M. & Lynch, G., 1979. Nature, 232: 748–750.

    Google Scholar 

  39. Biziere, K., Thompson, H. & Coyle, J. T., 1980. Brain Res. 183: 421–433.

    PubMed  Google Scholar 

  40. Vincent, S. R. & McGeer, E. G., 1980. Brain Res. 184: 99–108.

    PubMed  Google Scholar 

  41. Head, R. A., Tunnicliff, G., Matheson, G. K., 1980. Can. J. Biochem. 58: 534–538.

    PubMed  Google Scholar 

  42. Sanderson, C. & Murphy, S., 1980. Dev. Neurosci, 9: 233–234.

    Google Scholar 

  43. De Barry, J., Vincendon, G. & Gombos, G., 1980. FEBS Letts. 109: 175–179.

    Google Scholar 

  44. Michaelis, E. K., 1975. Biochem. Biophys. Res. Comm. 65: 1004–1012.

    PubMed  Google Scholar 

  45. Lindstrom, J., Anholt, R., Einarson, B., Engel, A., Osame, M. & Montal, M., 1980. J. Biol. Chem. 255: 8340–8350.

    PubMed  Google Scholar 

  46. Wu, W. C. S. & Raftery, M. A., 1981. Biochemistry, 20: 694–701.

    PubMed  Google Scholar 

  47. Grubbs, R. D. & Michaelis, E. K., 1979. Soc. Neurosci. Abst. 5: 304.

    Google Scholar 

  48. Kuznetsov, V. I. & Mogilyanskii, D. N., 1975. Stud. Biophys. 49: 61–66.

    Google Scholar 

  49. Kolomytkin, O. V., Kuznetsov, V. I. & Ermishkin, L. N., 1979. Deposited Doc. VINITI 1672–1679.

  50. Kolomytkin, O. V., Kuznetsov, V. I. & Akoev, I. G., 1979. Zh. Biol. Khim. 1979 Abstr. 5F 46.

  51. De Robertis, E., 1975. Synaptic Receptors, Marcel Dekker, New York.

    Google Scholar 

  52. Levinson, S. R. & Keynes, R. D., 1972. Biochim. Biophys. Acta. 288: 241–247.

    PubMed  Google Scholar 

  53. Donnellan, J. F., Jewess, P. J. & Cattell, K. J., 1975. J. Neurochem. 25: 623–629.

    PubMed  Google Scholar 

  54. Taylor, R. F., 1978. J. Neurochem, 31: 1183–1198.

    PubMed  Google Scholar 

  55. De Robertis, E. & Fiszer de Plazas, S., 1976. J. Neurochem. 26: 1237–1243.

    PubMed  Google Scholar 

  56. Lunt, G. G., 1973. Comp. Gen. Pharmacol. 4: 75–79.

    Google Scholar 

  57. James, R. W., Lunt, G. G. & Donnellan, J. F., 1977. Insect Biochem. 7: 247–255.

    Google Scholar 

  58. James, R. W., Lunt, G. G. & Donnellan, J. F., 1974. Abst. Commun. 9th Mtg. Fed. Europ. Biochem. Soc. Hungary. p. 258.

  59. Fiszer de Plazas, S., De Robertis, E. & Lunt, G. G., 1977. Gen. Pharmac. 8: 133–137.

    Google Scholar 

  60. Fiszer de Plazas, S. & De Robertis, E., 1973. FEBS Letts. 33: 45–48.

    Google Scholar 

  61. Fiszer de Plazas, S. & De Robertis, E., 1974. J. Neurochem. 23: 1115–1120.

    PubMed  Google Scholar 

  62. James, R. W., Lunt, G. G. & Donnellan, J. F., 1977. Biochem. Soc. Trans. 5: 170–172.

    PubMed  Google Scholar 

  63. Briley, P. A., Filbin, M. T., Lunt, G. G. & Donnellan, J. F., 1980. In Insect Neurobiology and Pesticide Action, pp. 177–184, Soc. Chem. Industry, London.

    Google Scholar 

  64. Cleworth, J. F., Robinson, N. L. & Usherwood, P. N. R., 1980. In Insect Neurobiology and Pesticide Action pp. 280–281, Soc. Chem. Industry, London.

    Google Scholar 

  65. Cull-Candy, S. G. & Usherwood, P. N. R., 1973. Nature, 246: 62–64.

    Google Scholar 

  66. Cull-Candy, S. G. & Usherwood, P. N. R., 1976. J. Physiol. (London) 255: 449–464.

    Google Scholar 

  67. Cull-Candy, S. G. & Usherwood, P. N. R., 1974. Neuropharmacol 13: 455–461.

    Google Scholar 

  68. Krogsgaard-Larsen, P., Honore, T., Hansen, J. J., Curtis, D. R. & Lodge, D., 1980. Nature, 284: 64–66.

    PubMed  Google Scholar 

  69. Krogsgaard-Larsen, P., Honore, T., Hansen, J. J., Curtis, D. R. & Lodge, D., 1981. In Glutamate as a Neurotransmitter (Di Chiara, G. & Gessa, G. L., eds.) pp. 285–294, Raven Press, New York.

  70. Honore, T., Lauridsen, J. & Krogsgaard-Larsen, P., 1981. J. Neurochem. 36: 1302–1304.

    PubMed  Google Scholar 

  71. Cull-Candy, S. G., Donnellan, J. F., James, R. W. & Lunt, G. G., 1976. 262: 408–409.

  72. Bioulac, B., De Tinguy-Moreaud, E., Vincent, J.-D. & Neuzil, E., 1979. Gen. Pharmacol. 10: 121–125.

    PubMed  Google Scholar 

  73. Watkins, J. C., Curtis, D. R. & Brand, S. S., 1977. J. Pharm. Pharmacol. 29: 324.

    PubMed  Google Scholar 

  74. Davies, J., Evans, R. H., Francis, A. A., Jones, A. W. & Watkins, J. C., 1980. In Neurotransmitters and their Receptors (Littauer, U. Z., Dudai, Y., Silman, I., Teichberg, V. I. & Vogel, Z., eds.) pp. 333–347, John Wiley and Sons Ltd., London.

  75. Johnston, G. A. R., 1979. In Glutamic Acid: Advances in Biochemistry and Physiology (Filer, L. J., et al., eds.) pp. 177–185, Raven Press, New York.

  76. Snodgrass, S. R., 1979. Soc. Neurosci. Abst. 5: 572.

    Google Scholar 

  77. Walther, C., 1980. In Insect Neurobiology and Pesticide Action, pp. 153–160, Soc. Chem. Industry, London.

    Google Scholar 

  78. May, T. E. & Pick, T., 1979. J. Insect. Physiol. 25: 685–691.

    PubMed  Google Scholar 

  79. Peik, T., Mantel, P. & Jas, 1980. J. Insect Physiol. 26: 345–349.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Biochemistry, Bath University, BA2 7AY, Bath, UK

    P. A. Briley, M. T. Filbin, G. G. Lunt & P. D. Turner

Authors
  1. P. A. Briley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. T. Filbin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. G. Lunt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. D. Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Briley, P.A., Filbin, M.T., Lunt, G.G. et al. Glutamate receptor binding in insects and mammals. Mol Cell Biochem 39, 347–356 (1981). https://doi.org/10.1007/BF00232584

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232584

Keywords

Search

Navigation