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Optimizing sweet potato [Ipomoea batatas (L.) Lam.] root and plantlet formation by selection of proper embryo developmental stage and size, and gel type for fluidized sowing

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Abstract

Potassium starch polyacrylamide, potassium acrylate, a copolymer of potassium acrylate and acrylamide, and hydroxyethylcellulose carrier gels were tested to find a fluid drilling material suited for synthetic seeding of sweet potato (Ipomoea batatas (L.) Lam.) somatic embryos. Somatic embryo developmental stage and size, and maturation (incubation) time were also evaluated to improve plantlet formation. All embryos suspended in the fluidized hydroxyethylcellulose gel were viable after six days and 7% developed into plantlets after two weeks. Up to 97% of the somatic embryos suspended in acrylate and/or acrylamide gels died within six days. Root development was at least 10% and plantlet development at least 30% greater when embryos were subcultured on basal medium for 16 instead of 25 days prior to placement and suspension in hydroxyethylcellulose gel. Up to 25% more plantlets were obtained from embryos at the elongated torpedo stage than those at the cotyledonary or torpedo stages of development. When suspended in hydroxyethylcellulose gel embryo length had no effect on the percentage of plantlets obtained.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog medium (1962)

PC:

copolymer of potassium acrylate and acrylamide

PSA:

potassium starch polyacrylamide

PA:

potassium acrylate

HEC:

hydroxyethylcellulose

ODR:

oxygen diffusion rate

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Author notes

  1. Jonathan R. Schultheis

    Present address: Horticultural Science Department, North Carolina State University, Box 7609, 27695-7609, Raleigh, NC, USA

Authors and Affiliations

  1. Vegetable Crops Department, University of Florida, 32611, Gainesville, FL, USA

    Jonathan R. Schultheis, Daniel J. Cantliffe & Raymond P. Chee

Authors
  1. Jonathan R. Schultheis
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  2. Daniel J. Cantliffe
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  3. Raymond P. Chee
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Additional information

Communicated by C. T. Harms

Florida Agr. Expt. Sta., Journal Series No. R-00253 Mention of proprietary products is for convenience of reader only, and does not constitute endorsement by the University of Florida

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Schultheis, J.R., Cantliffe, D.J. & Chee, R.P. Optimizing sweet potato [Ipomoea batatas (L.) Lam.] root and plantlet formation by selection of proper embryo developmental stage and size, and gel type for fluidized sowing. Plant Cell Reports 9, 356–359 (1990). https://doi.org/10.1007/BF00232398

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  • DOI: https://doi.org/10.1007/BF00232398

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