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Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds

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Abstract

Green Protocorm-like Bodies (PLB) with high multiplication capacity were induced from shoot tips of flower stalk buds having 1 or 2 leaf primordia using New Dogashima Medium (NDM) containing 0.1 mg l−1 α-naphthaleneacetic acid (NAA) and 1 mg 1−1 6-benzylaminopurine (BAP). These PLB were subcultured on the same medium. More than 10,000 PLBs were obtained from a few buds on a single flower stalk within one year. After transfer onto NDM containing no plant growth regulator (PGR), the PLB developed into plantlets. The micropropagation method formulated in this study was applicable to 12 different genotypes. These results suggest that the methodology could be used on a commercial scale for vegetative propagation of Phalaenopsis and Doritaenopsis.

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Abbreviations

PLB:

protocorm-like body

PGR:

plant growth regulator

NAA:

α-naphthaleneacetic acid

BAP:

6-benzylaminopurine

NDM:

New Dogashima Medium

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Authors and Affiliations

  1. Research and Development Center, Orchid Sanctuary Dogashima, Nishina, Nishiizu-cho, Kamo-gun, 410-35, Shizuoka, Japan

    Ken Tokuhara

  2. Faculty of Horticulture, Chiba University, Matsudo, 271, Chiba, Japan

    Masahiro Mii

Authors
  1. Ken Tokuhara
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  2. Masahiro Mii
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Additional information

Communicated by G. C. Phillips

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Tokuhara, K., Mii, M. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Reports 13, 7–11 (1993). https://doi.org/10.1007/BF00232306

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  • DOI: https://doi.org/10.1007/BF00232306

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