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Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants

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Abstract

A procedure for rapid multiplication of Chrysanthemum morifolium RAMAT cv. Birbal Sahni using leaf callus and stem (nodal/internodal) callus as well as node and apical shoots has been developed. Murashige and Skoog's medium (1962) supplemented with 2mg/1 2,4-D yielded good green calli from both leaf and stem segments within 2 weeks. About 1 cm × 1 cm callus regenerated 2–3 shoots after 3 weeks on MS solid medium supplemented with 0.1 mg/l IAA and 0.2 mg/l BAP. Each of the regenerated shoots when transferred to the same shooting medium without agar yielded about 150 new shoots, which in turn regenerated roots after another week in MS half strength or modified White's media (Rangaswamy, 1961). It has been estimated that about 1014 plantlets could be produced in a year from one expiant following the proposed protocol.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog's (1962) medium

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Authors and Affiliations

  1. Biotechnology Unit, Department of Chemical Engineering, I.I.T., 721 302, Kharagpur, India

    Parthasarathi Bhattacharya, Satyahari Dey, Nilanjana Das & Bimal Chandra Bhattacharyya

Authors
  1. Parthasarathi Bhattacharya
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  2. Satyahari Dey
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  3. Nilanjana Das
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  4. Bimal Chandra Bhattacharyya
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Additional information

Communicated by A. R. Gould

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Bhattacharya, P., Dey, S., Das, N. et al. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Reports 9, 439–442 (1990). https://doi.org/10.1007/BF00232268

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  • DOI: https://doi.org/10.1007/BF00232268

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