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Agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants

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Abstract

Transformed calli and shoots of strawberry (Fragaria × ananassa Duch.) cv. Redcoat were obtained using Agrobacterium tumefaciens carrying plasmid pB1121. Inoculated leaf explants produced transgenic calli at a frequency of 3% on selection medium containing 50 μg/ml kanamycin. Twenty per cent of selected caili regenerated, giving rise to transgenic shoots. All transgenic calli and shoots expressed substantial amounts of GUS and NPT-II activity. The Southern blot analysis confirmed the insertion of both marker genes into the strawberry genome as single and multiple copy inserts. The transgenic shoots elongated on rooting medium in the presence of 25 μg/ml kanamycin, but exhibited reduced rooting ability.

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Abbreviations

BA:

benzyladenine

NAA:

1-naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

indole-3-butyric acid

NPT-II:

neomycin phosphotransferase(EC 2.7.1.95)

GUS:

β-glucuronidase(EC 3.2.1.31)

X-GLUC:

5-bromo-4-chloro-3-indolyl-glucuronide

4-MU:

4-methylumbelliferone

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Author information

Author notes

  1. Cecil Stushnoff

    Present address: Department of Biochemistry, Colorado State University, Fort Collins, CO, USA

Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Road, Saskatoon, S7N OW9, Saskatchewan, Canada

    Ravindra N. Chibbar, Kutty K. Kartha, Raju S. S. Datla & William L. Crosby

  2. Department of Horticulture Science, University of Saskatchewan, Saskatoon, S7N OWO, Saskatchewan, Canada

    Narender S. Nehra & Cecil Stushnoff

Authors
  1. Narender S. Nehra
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  2. Ravindra N. Chibbar
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  3. Kutty K. Kartha
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  4. Raju S. S. Datla
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  5. William L. Crosby
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  6. Cecil Stushnoff
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Additional information

Communicated by F. Constabel

NRCC No. 31227

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Nehra, N.S., Chibbar, R.N., Kartha, K.K. et al. Agrobacterium-mediated transformation of strawberry calli and recovery of transgenic plants. Plant Cell Reports 9, 10–13 (1990). https://doi.org/10.1007/BF00232125

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  • DOI: https://doi.org/10.1007/BF00232125

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