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Coat protein mediated resistance to Plum Pox Virus in Nicotiana clevelandii and N. benthamiana

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Abstract

Transgenic Nicotiana benthamiana and N. clevelandii plants expressing the coat protein of Plum Pox Virus under the control of the 35S promoter from Cauliflower Mosaic Virus were engineered by Agrobacterium tumefaciens mediated transformation. The phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with Plum Pox Virus. N. clevelandii coat protein transgenic plants circumvent virus accumulation. After an initial increase in virus titer similar to the control plants, some coat protein expressing plants showed a reduced accumulation of virus and inhibition of the systemic spread, characterized by decrease of the virus titer and formation of new symptomless leaves. In other N. clevelandii coat protein expressing plants virus accumulation was inhibited and disease symptoms never appeared. N. benthamiana coat protein expressing plants were also protected. After a temporary virus accumulation, virus titer decreased without the appearance of symptoms with the exception of a few plants, which showed a delay of thirty days in the development of symptoms post challenge infection.

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Abbreviations

PPV:

Plum Pox Virus

CP:

coat protein

CaMV:

Cauliflower Mosaic Virus

CP+:

coat protein expressing plant

CP−:

control plant = non coat protein expressing plant

TMV:

Tobacco Mosaic Virus

NPTII:

neomycin phosphotransferaseII

IBA:

indole-3-butyric acid

BAP:

6-benzylaminopurine;

MS:

Murashige Skoog

ELISA:

enzyme linked immunosorbent assay

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Authors and Affiliations

  1. University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria

    Ferdinand Regner, Artur da Câmara Machado, Margit Laimer da Câmara Machado, Herta Steinkellner, Diethard Mattanovich, Veronika Hanzer, Hans Weiss & Hermann Katinger

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  1. Ferdinand Regner
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  2. Artur da Câmara Machado
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  3. Margit Laimer da Câmara Machado
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  4. Herta Steinkellner
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  5. Diethard Mattanovich
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  6. Veronika Hanzer
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  7. Hans Weiss
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  8. Hermann Katinger
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Communicated by H. Lörz

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Regner, F., da Câmara Machado, A., da Câmara Machado, M.L. et al. Coat protein mediated resistance to Plum Pox Virus in Nicotiana clevelandii and N. benthamiana . Plant Cell Reports 11, 30–33 (1992). https://doi.org/10.1007/BF00231835

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  • DOI: https://doi.org/10.1007/BF00231835

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