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Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus

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Summary

A system was developed which allows the transfer of foreign genes into apricot cultivars. We report the transformation and regeneration of Prunus armeniaca plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene ß-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV). The marker gene GUS was used for optical evaluation of the efficiency of the transformation system. The coat protein gene of PPV was used to introduce coat protein mediated resistance against one of the most important pathogens of stone fruit trees in Europe and the whole Mediterranean area. This is the first report of the successful integration of a viral coat protein gene into a fruit tree species, opening a new perspective on the control of the disease.

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Abbreviations

GUS:

ß-glucuronidase

PPV:

Plum Pox Virus

BA:

6-benzylaminopurine

NPTII:

neomycin phosphotransferase II

CP:

coat protein

CaMV:

Cauliflower Mosaic Virus

P35S 35S:

promoter

MS:

Murashige and Skoog

PCR:

polymerase chain reaction

P/C/I:

phenol/chloroform/isoamylalcohol

RNase:

ribonuclease

dNTP:

deoxyribonucleosidetriphosphate

DMSO:

dimethyl sulfoxide

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Authors and Affiliations

  1. University of Agriculture and Forestry, Institute of Applied Microbiology, Nußdorfer Lände 11, 1190, Vienna, Austria

    Margit Laimer da Câmara Machado, Artur da Câmara Machado, Veronika Hanzer, Hans Weiss, Ferdinand Regner, Herta Steinkellner, Diethard Mattanovich, Regina Plail, Elisabeth Knapp, Birgit Kalthoff & Hermann Katinger

Authors
  1. Margit Laimer da Câmara Machado
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  2. Artur da Câmara Machado
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  3. Veronika Hanzer
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  4. Hans Weiss
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  5. Ferdinand Regner
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  6. Herta Steinkellner
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  7. Diethard Mattanovich
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  8. Regina Plail
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  9. Elisabeth Knapp
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  10. Birgit Kalthoff
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  11. Hermann Katinger
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Additional information

Communicated by H. Lörz

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da Câmara Machado, M.L., da Câmara Machado, A., Hanzer, V. et al. Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus. Plant Cell Reports 11, 25–29 (1992). https://doi.org/10.1007/BF00231834

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  • DOI: https://doi.org/10.1007/BF00231834

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