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Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens

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Abstract

Leaf and stem explants of passionfruit (Passiflora eadulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tunefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-solidified Murashige and Skoog (1962) based medium containing 4.43 μM 6-benzyl-aminopurine and supplemented with 86 μM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14.7 μM 3-indolebutyric acid and 2.68 μM α-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 μM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants.

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Abbreviations

BAP:

6-benzylaminopurine

CTAB:

hexadecy-Itrimethylammonium bromide

EDTA:

ethylenediaminetetraacetic acid

IBA:

3-indolebutyric acid

MS:

Murashige and Skoog (1962)

NAA:

α-naphthyleneacetic acid

NPTII:

neomycin phosphotransferase II

nptII:

neomycin phosphotransferase II gene

PCR:

polymerase chain reaction

SDS:

sodium dodecyl sulphate

Tris:

tris(hydroxymethyl)aminomethane

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Author notes

  1. G. Manders

    Present address: Department of Forest Genetics, Swedish University of Agriculture, P.O. Box 7027, S-75007, Uppsala, Sweden

Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, NG7 2RD, Nottingham, UK

    G. Manders, W. C. Otoni, F. B. d'Utra Vaz, N. W. Blackball, J. B. Power & M. R. Davey

Authors
  1. G. Manders
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  2. W. C. Otoni
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  3. F. B. d'Utra Vaz
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  4. N. W. Blackball
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  5. J. B. Power
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  6. M. R. Davey
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Additional information

Communicated by H. Lörz

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Manders, G., Otoni, W.C., d'Utra Vaz, F.B. et al. Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens . Plant Cell Reports 13, 697–702 (1994). https://doi.org/10.1007/BF00231627

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  • DOI: https://doi.org/10.1007/BF00231627

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