Summary
A highly efficient method for stable wheat transformation using hygromycin resistance as a selectable marker is described. Young embryogenic calli growing from immature wheat embryos were transformed using a gunpowder-driven microparticle accelerator. Transgenic wheat plants were determined by PCR amplification of transgene fragments and confirmed by Southern hybridization, activity of the transgene expression and by analysis of the progeny. The hpt gene was as good as or a better selectable marker than the bar gene with an average efficiency (number of transgenic plants relative to the number of bombarded calli) of 5.5% compared with 2.6% for the bar gene.
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References
Becker D, Brettschneider R, Lörz H (1994) Plant J 5:299–307
Datta SK, Peterhans A, Datta K, Potrykus I (1990) Biotechnology 8:736–740
De Block M, Botterman J, Vandewiele M, Dockx J, Thoen C, Gossele V, Rao Movva N, Thompson C (1987) EMBO J 6:2513–2518
Edwards K, Johnstone C, Thompson C (1991) Nucl Acids Res 19:1349
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM (1990) Biotechnology 8:833–839
Jefferson RA (1987) Plant Mol Biol Reporter 5:387–405
Klein TM, Gradziel T, Fromm ME, Sanford JC (1988) Biotechnology 6:559–563
Li Z, Burow MD, Murai N (1990) Plant Mol Biol Reporter 8:276–291
McElroy D, Brettell RIS (1994) Tibtech 12:62–68
Murashige T, Skoog F (1962) Physiol Plant 15:473–476
Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Plant J 5:285–297
Potrykus I (1990) Biotechnology 8:535–542
Reggiardo MI, Arana JL, Orsaria LM, Permingeat HR, Spitteler MA, Vallejos RH (1991) Plant Sci 75:237–243
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Proc Natl Acad Sci USA 81:8014–8018
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, Vol 2, Cold Spring Harbor, New York
Shimamoto K, Terada R, Izawa T, Fugimoto H (1989) Nature 338:274–276
Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Biotechnology 10:667–674
Vasil V, Srivastava V, Castillo AM, Fromm ME, Vasil IK (1993) Biotechnology 11:1553–1558
Weeks JT, Anderson OD, Blechl AE (1993) Plant Physiol 102:1077–1084
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Communicated by G. C. Phillips
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Ortiz, J.P.A., Reggiardo, M.I., Ravizzini, R.A. et al. Hygromycin resistance as an efficient selectable marker for wheat stable transformation. Plant Cell Reports 15, 877–881 (1996). https://doi.org/10.1007/BF00231579
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DOI: https://doi.org/10.1007/BF00231579