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Quantitative and localized element analysis in cross-sections of spruce [Picea abies (L.) Karst.] needles with different degrees of damage

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Summary

Proton-induced X-ray microanalysis (micro-PIXE) permits the simultaneous determination of the content and the distribution of elements with atomic numbers higher than Z = 13 in biological samples. This method was used to investigate element content and localization in cross-sections of 6-month-old spruce needles. It was possible to detect the elements silicon, phosphorus, sulphur, chlorine, potassium, calcium, manganese, iron and zinc in semithin (10 μm) sections of the needles. The localization of the cationic elements like potassium, calcium and manganese was determined in the one-dimensional line scan mode and in the two-dimensional raster scan mode. To demonstrate the usefulness of this method for forest decline research, element content and localization were compared in needles from two trees, which differed in their degree of damage. We were able to detect differences in the amount of cations and in their distribution inside the needles.

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Godde, D., Divoux, S., Höfert, M. et al. Quantitative and localized element analysis in cross-sections of spruce [Picea abies (L.) Karst.] needles with different degrees of damage. Trees 5, 95–100 (1991). https://doi.org/10.1007/BF00227491

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