Summary
The Cytoplasmic Male Sterility (CMS)-associated region in Petunia, the S-Pcf locus, was defined by the analysis of recombinant mitochondrial genomes of somatic hybrid plants resulting from a fusion of protoplasts from CMS and fertile lines. The presence of the S-Pcf locus was shown to correlate with the CMS trait in stable somatic hybrids and in other CMS Petunia lines. A small population of unstable, sterile somatic hybrids was also generated in this fusion, most of which underwent cytoplasmic segregation in subsequent generations. Stable revertants of such sterile somatic hybrids were shown to lose the S-Pcf locus. In this paper we present a molecular and genetic analysis of unstable progenies of an unstable, sterile somatic hybrid plant derived from the same fusion experiment. Both male-sterile and fertile progenies of this somatic hybrid plant have shown continuous segregation of fertile and male-sterile progenies. All segregants in this line contained, and transcribed, the S-Pcf locus. Genetic analysis indicated the presence of various levels of multiple nuclear fertility-restoration genes in this group of progenies. These findings consolidate the association between the S-Pcf locus and the CMS trait in Petunia. It also shows that the restoration of fertility by the multiple nuclear gene system does not affect the transcription of the S-Pcf locus and that the presence of an intact S-Pcf locus is necessary in order to maintain the potential sterility in the cytoplasm.
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References
Boeshore MI, Hanson MR, Izhar S (1985) A variant mitochondrial DNA arrangement specific to Petunia stable sterile somatic hybrids. Pl Mol Biol 4:125–132
Clark E, Gafni Y, Izhar S (1988) Loss of CMS-specific mitochondrial DNA arrangement in fertile revertants of Petunia. Pl Mol Biol 11:249–253
Ettinger-Paltin R (1981) Growth hormones as selection tool for somatic hybridization in Petunia. MSc thesis, Hebrew University of Jerusalem, Israel (in Hebrew, with English summary)
Feinberg A, Vogelstein B (1983) A technique for labeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–11
Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–265
Hanson MR, Pruitt KD, Nivison HT (1989) Male sterility loci in plant mitochondrial genomes. Oxford Surveys Plant Mol Cell Biol 6:61–85
Izhar S (1978) Cytoplasmic male sterility in Petunia. III. Genetic control of microsporogenesis and male fertility restoration. J Hered 69:22–26
Izhar S (1984) Male sterility in Petunia. In: Sink KC (ed) Monographs on theoretical and applied genetics, vol 9, Petunia. Springer-Verlag, New York, pp 77–91
Izhar S, Frankel R (1976) Cytoplasmic male sterility in Petunia. I. Comparative study of different plasmatype sources. J Hered 67:43–46
Izhar S, Schlicter M, Swartzberg D (1983) Sorting out of cytoplasmic elements in somatic hybrids of Petunia and the prevalence of the heteroplasmon through several meiotic cycles. Mol Gen Genet 190:468–474
Lifshitz I (1986) Isolation and characterization of the mitochondrial genome in Petunia. MSc thesis, Hebrew University of Jerusalem, Israel (in Hebrew, with English summary)
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Murray HG, Thompson WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4325
Nivison HT, Hanson MR (1989) Identification of a mitochondrial protein associated with cytoplasmic male sterility in petunia. The Plant Cell 1:1121–1130
Pring DR, Lonsdale DM (1985) The higher plant mitochondrial genome. Int Rev Cytol 97:1–46
Rasmussen J, Hanson MR (1989) A NADH dehydrogenase subunit gene is co-transcribed with the abnormal Petunia mitochondrial gene associated with cytoplasmic male sterility. Mol Gen Genet 215:332–336
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251
Schlicter M (1983) Genetic analysis of somatic hybrids in Petunia: cytoplasmic segregation of normal and (S) plasmons. MSc thesis, Hebrew University of Jerusalem, Israel (in Hebrew, with English summary)
Wolf O, Clark E, Soferman O, Izhar S (1988) A unique mitochondrial gene region associated with cms in Petunia. 2nd Int Congress Plant Molec Biol: abs # 347
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49
Young EG, Hanson MR, Dierks PM (1986) Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit. Nucleic Acids Res 14:7995–8006
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Communicated by Yu. Gleba
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Wolf-Litman, O., Soferman, O., Tabib, Y. et al. Interaction of the mitochondrial S-Pcf locus for cytoplasmic male sterility in Petunia with multiple fertility-restoration genes in somatic hybrid plants. Theoret. Appl. Genetics 84, 829–834 (1992). https://doi.org/10.1007/BF00227392
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DOI: https://doi.org/10.1007/BF00227392