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Integration of lipid metabolism in the mammary gland and adipose tissue by prolactin during lactation

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Abstract

Prolactin deficiency, induced by bromocryptine treatment, brought about reciprocal changes in the ability of adipocytes and acini isolated from lactating rats to synthesize lipids. The capacity to synthesize fatty acids and phospholipids decreased in the mammary gland and increased in adipocytes by bromocryptine treatment. In the mammary gland, the maximum potential activity of the pentose shunt as well as the specific activities of the pathway dehydrogenases were significantly reduced by bromocryptine treatment. Simultaneously, adipose tissue increased its lipogenic capacity but neither the maximum potential of the shunt nor the specific activities of the pentose phosphate shunt dehydrogenases were significantly changed with respect to the control lactating rats. Thus, a differential regulatory mechanism(s) of the pentose phosphate shunt activity appears to operate in these two tissues. Adipocytes from lactating rats showed a poor responsiveness to insulin in terms of lipid synthesis from glucose. In contrast, in adipocytes from bromocryptine treated rats insulin was able to increase lipid synthesis (105%). Sheep prolactin administration ‘in vivo’ partially reversed the effects of bromocryptine. These data suggest that prolactin mediates adipocytes resistance to insulin during lactation. Phospholipid synthesis, as occurred in fatty acid synthesis, is increased in adipose tissue and decreased in mammary gland by bromocryptine treatment. However, α-adrenergic stimulation increases phosphatidylinositol turnover to about the same percentages in both mammary gland acini and adipocytes from lactating rats independently of bromocryptine treatment.

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Author notes

  1. Manuel Ros

    Present address: Departamento de Quimica, Universidad de Castilla-La Mancha, 13071, Cuidad Real, Spain

Authors and Affiliations

  1. Departamento de Biología Molecular, Centro de Biología Molecular (U.A.M.-C.S.I.C.), Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Manuel Ros, María F. Lobato, Josefa P. García-Ruíz & Francisco J. Moreno

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  1. Manuel Ros
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  2. María F. Lobato
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  3. Josefa P. García-Ruíz
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  4. Francisco J. Moreno
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Ros, M., Lobato, M.F., García-Ruíz, J.P. et al. Integration of lipid metabolism in the mammary gland and adipose tissue by prolactin during lactation. Mol Cell Biochem 93, 185–194 (1990). https://doi.org/10.1007/BF00226191

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  • DOI: https://doi.org/10.1007/BF00226191

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