Abstract
The quality of plant oil is determined by its component fatty acids. Relatively high levels of linolenic acid reduce the oxidative stability of the oil, and high levels of erucic acid in the diet have been associated with health problems. Thus, oilseed Brassica napus cultivars with low linolenic and low erucic acid contents are highly desirable for edible oil production. In order to identify genes controlling the levels of erucic and linolenic acids, we analyzed the oil composition of 99 F1-derived doubled haploid lines from a cross between cv ‘Major’ (high levels of erucic and linolenic acids) and cv ‘Stellar’ (low levels of both fatty acids). A molecular marker linkage map of 199 loci for this population was used to identify quantitative trait loci (QTL) controlling oil composition. We identified two regions that accounted for nearly all of the phenotypic variation in erucic acid concentration and one region that accounted for 47% of the variation in linolenic acid concentration. The QTL associated with linolenic acid concentration mapped near a RFLP locus detected by a cDNA clone encoding an omega-3 desaturase, suggesting that the low linolenic acid content of ‘Stellar’ may be due to a mutation in this gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, Somerville CR (1992) Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258:1353–1355
Auld DL, Heikkinen MK, Erickson DA, Sernyk JL, Romero JE (1992) Rapeseed mutants with reduced levels of polyunsaturated fatty acids and increased levels of oleic acid. Crop Sci 32:657–662
Beare JI, Campbell JA, Youngs CG, Craig BM (1963) Effects of saturated fat in rats fed rapeseed oil. Can J Biochem Physiol 41:605–612
Chen BY, Haneem WK, Simonsen V (1989) Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L. B. campestris L., and B. alboglabra Bailey. Theor Appl Genet 77:673–679
Christie WW (1982) Lipid analysis, 2nd edn. Pergamon Press, Sydney
Downey RK, Rakow GFW (1987) Rapeseed and mustard. In: Fehr WR (ed) Principles of cultivar development, vol 2. Macmillan, New York, pp 437–486
Ferreira ME, Williams PH, Osborn TC (1994) RFLP mapping of Brassica napus using doubled haploid lines. Theor Appl Genet 89:615–621
Ferreira ME, Williams PH, Osborn TC (1995a) Mapping of a locus controlling resistance to Albugo Candida in Brassica napus using molecular markers. Phytopathology 85:218–220
Ferreira ME, Williams PH, Osborn TC (1995b) Mapping loci controlling Brassica napus resistance to Leptosphaeria maculons under different screening conditions. Phytopathology 85:213–217
Ferreira ME, Satagopan J, Yandell B, Williams PH, Osborn TC (1995c) Mapping loci controlling vernalization requirement and flowering time in Brassica napus. Theor Appl Genet 90:727–732
Harvey BL, Downey RK (1964) The inheritance of erucic acid content in rapeseed (Brassica napus). Can J Plant Sci 44:104–111
Hu J, Quiros C, Arus P, Struss D, Robbelen G (1995) Mapping of a gene determining linolenic acid concentration in rapeseed with DNA-based markers. Theor Appl Genet 90:258–262
Jonsson R (1977) Erucic-acid heredity in rapeseed (Brassica napus L. and Brassica campestris L.). Hereditas 86:159–170
Kondra ZP, Stefansson BR (1965) Inheritance of erucic and eicosenoic acid content of rapeseed oil (Brassica napus). Can J Genet Cytol 7:505–510
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenet 12:172–175
Krzymanski J, Downey RK (1969) Inheritance of oleic, linoleic and linolenic acids in seed oil of rapeseed, Brassica napus. Can J Plant Sci 55:205–210
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Lander E, Green P, Abrahanson J, Barlow A, Daley M. Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Laryea MD, Jiang YF, Xu GL (1992) Fatty acid composition of blood lipids in Chinese children consuming high erucic acid rapeseed oil. Ann Nutr Met 36:273–278
Lincoln S, Daly M,Lander E (1992) Mapping genes controlling quantitative traits using MAPMAKER/QTL version 1.1: a tutorial and reference manual, 2nd edn. WhiteHead Institute for Biomedical Research Technical Report, Cambridge, Mass
Ott J (1992) Analysis of human genetic linkage. The Johns Hopkins University Press, Baltimore
Princen LH, Rothfus JA (1984) Development of new crops for industrial raw materials. J Am Oil Chem Soc 61:281–289
Robbelen G, Nitsch A (1975) Genetical and physiological investigations on mutants for polyenoic fatty acids in rapeseed, Brassica napus L. Z Pflanzenzucht 75:93–105
Scarth R, McVetty PBE, Rimmer SR, Stefansson BR (1988) ‘Stellar’ low linolenic-high linoleic acid summer rape. Can J Plant Sci 68:509–511
Somerville C, Browse J (1991) Plant lipids: metabolism, mutants, and membranes. Science 252:80–87
Teutonico RA, Osborn TC (1994) Mapping of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89:885–894
Thormann CE, Ferreira ME, Camargo LEA, Tivang JG, Osborn TC (1994) Comparison of RFLP and RAPD markers for estimating genetic relationships within and among cruciferous species. Theor Appl Genet 88:973–980
Author information
Authors and Affiliations
Additional information
Communicated by G. E. Hart
Rights and permissions
About this article
Cite this article
Thormann, C.E., Romero, J., Mantet, J. et al. Mapping loci controlling the concentrations of erucic and linolenic acids in seed oil of Brassica napus L.. Theoret. Appl. Genetics 93, 282–286 (1996). https://doi.org/10.1007/BF00225758
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225758