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Adenylate cyclase activity in Xenopus laevis ovarian follicles

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Summary

An adenylate cyclase activity was identified and characterized in preparations from Xenopus laevis ovarian tissue and follicles. The enzyme is more active in the presence of Mn2+ than of Mg2+, and it is highly activated by fluoride, guanyl-5′-yl-imidodiphosphate [Gpp(NH)p] and cholera toxin. During the last stages of oogenesis, as the oocytes grow in volume, the total adenylate cyclase activity increases more than tenfold, maintaining a constant relationship to the surface area of the follicle.

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Authors and Affiliations

  1. Departamento de Bioquímica, Facultad de Medicina, and Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 6671, 7, Santiago, Chile

    Xavier Jordana, Carlos Otero, Catherine C. Allende, Jorge Allende, Mirtha M. Flawia, Alberto R. Kornblihtt & Hector N. Torres

  2. Instituto de Investigaciones Bioquímicas ‘Fundación Campoma’ and Facultad de Ciencias Exactas y Naturales, Obligado 2490, 1428, Buenos Aires, Argentina

    Xavier Jordana, Carlos Otero, Catherine C. Allende, Jorge Allende, Mirtha M. Flawia, Alberto R. Kornblihtt & Hector N. Torres

Authors
  1. Xavier Jordana
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  2. Carlos Otero
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  3. Catherine C. Allende
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  4. Jorge Allende
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  5. Mirtha M. Flawia
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  6. Alberto R. Kornblihtt
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  7. Hector N. Torres
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Additional information

M. M. F. and H. N. T. are Career Investigators of the Consejo Nacional de Investigaciones Cientificas y Técnicas (Argentina); A. R. K. is a fellow of the same institution.

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Jordana, X., Otero, C., Allende, C.C. et al. Adenylate cyclase activity in Xenopus laevis ovarian follicles. Mol Cell Biochem 40, 87–91 (1981). https://doi.org/10.1007/BF00224751

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  • DOI: https://doi.org/10.1007/BF00224751

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