Abstract
Crosses were made between Fanny (highly susceptible to blast) and 11 cultivars differing in blast resistance. Using the pedigree method (PM) segregating generations were evaluated and selected for blast resistance. Via anther culture (AC), doubled-haploids were obtained from F1 plants and from F2 blast-susceptible plants. Pedigree and anther culture-derived lines were planted together and evaluated for blast resistance under rainfed conditions at the Santa Rosa Experiment Station, Villavicencio, Colombia. The principal objective was to compare PM and AC in terms of their efficiency in producing rice lines resistant to blast. Results of a stratified analysis showed an association between method and blast resistance. Results of the logit-model analysis showed that AC produced a significantly (P=0.0001) higher proportion of lines with initial blast resistance (leaf- and neck-blast reaction ≤4) than did PM across all cross types. Stable blast resistance was assessed based on field performance over 3 years. AC was superior to PM in generating stable resistance for only some cross types. Consequently, with a few exceptions, AC can be used as effectively as PM to develop rice cultivars resistant to blast, with savings in time and labor. Additionally, blast-resistant lines were obtained either by the pedigree method or by anther culture from crosses between blast-susceptible cultivars (Fanny/CICA4 and Fanny/Colombial). This excludes somaclonal variation as a possible mechanism responsible for this resistance and suggests that a recombination of minor genes could have occurred and was fixed through either method. However, the stability of the resistance was greater in pedigree-derived lines. The implications of these findings for rice blast-resistance breeding are discussed.
Similar content being viewed by others
References
Baenziger PS, Schaeffer GW (1983) Dihaploids via anthers cultured in vitro. In: Owens LD (ed) Genetic engineering: applications to agriculture. Bestville Symposia in Agricultural Research. Row-man and Allanheld, New Jersey, pp 269–284
Baenziger PS, Wesenberg DM, Smail DM, Alexander WL, Schaeffer GL (1989) Agronomic performance of wheat doubled-haploid lines derived from cultivars by anther culture. Plant Breed 103:101–109
Buddenhagen IW (1983) Disease resistance in rice. In: Lamberti F, Waller JM, Van der Graaff NA (ed) Durable resistance in crops. Plenum Press, New York, pp 401–428
Caligari PDS, Powell W, Jinks JL (1987) A comparison of inbred lines derived by doubled haploidy and single-seed descendent in spring barley (Hordeum vulgare). Ann Appl Biol 111:667–675
Chu CC (1982) Haploids in plant improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 129–158
Chuang JC, Ouyang JW, Chia H (1978) A set of potato media for wheat anther culture. In: Proc Symp Plant Tissue Culture. Science Press, Beijing, pp 51–56
Correa Victoria FJ, Zeigler RS (1993a) Field breeding for durable rice blast resistance in the presence of diverse pathogen populations. In: Jacobs Th, Parlevliet JE (eds) Durability of disease resistance. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 215–218
Correa Victoria FJ, Zeigler RS (1993b) Pathogenic variability in Pyricularia grisea at a rice blast “hot spot” breeding site in eastern Colombia. Plant Disease 77:1029–1035
Correa Victoria FJ, Zeigler RS (1995) Stability of partial and complete resistance in rice to Pyricularia grisea under rainfed upland conditions in eastern Colombia. Phytopathology 85:977–982
Correa Victoria FJ, Zeigler RS, Levy M (1994) Virulence characteristics of genetic families of Pyricularia grisea in Colombia. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. Commonwealth Agricultural Bureau International, Wallingford, UK, pp 211–230
Crill P, Hamm YS, Beachell HM (1981) The rice blast disease in Korea and its control with race prediction and gene rotation. Korean J Breed 13:106–114
Cuevas Pérez FE, Guimaraes EP, Berrio LE, González DI (1992) Genetic base of irrigated rice in Latin America and the Caribbean, 1971 to 1989. Crop Sci 32:1054–1059
Friedt W, Breun J, Zuchner S, Foroughi-Wehr B (1986) Comparative value of androgenetic doubled-haploid and conventionally selected spring barley lines. Plant Breed 97:56–63
Huang CS, Tsay HS, Chern CG, Chen CC, Yeh CC, Tseng TH (1988) Japonica rice breeding using anther culture. J Agric Res China 37:1–8
IRRI (1988) Standard evaluation system for rice 3rd. ed. International Rice Testing Program. IRRI. Los Baños, Laguna, Philippines.
Leal D, Dávalos A, Delgado H, Urueña E (1989) Dos nuevas variedades de arroz para el piedemonte llanero Oryzica Llanos 4 y Oryzica Llanos 5. Arroz 38(362): 11–21
Levy M, Romao J, Marchetti MA, Hamer JE (1991) DNA fingerprinting with a dispersed repeated sequence resolves pathotype diversity in the rice blast fungus. Plant Cell 3:95–102
Levy M, Correa Victoria FJ, Zeigler RS, Xu S, Hamer JE (1993) Genetic diversity of the rice blast fungus in a disease nursery in Colombia. Phytopathology 83: 1427–1433
Lynch PT, Finch RP, Davey MR, Cocking EC (1991) Rice tissue culture and its application. In: Khush GS, Toenniessen GH (eds) Rice biotechnology. Commonwealth Agricultural Bureau International, Wallingford, UK, pp 135–156
Mackill DJ, Bonman JM (1992) Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82:746–749
Mantel N, Haenszel W (1959) Statistical aspects of the analysis of data from retrospective studies of disease. J Natl Cancer Inst 22:719–748
McCouch SR, Nelson RJ, Tohme J, Zeigler RS (1994) Mapping of blast-resistance genes in rice. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. Commonwealth Agricultural Bureau International, Wallingford, UK, pp 167–186
Miah MAA, Earle ED, Khush GS (1985) Inheritance of callus formation ability in anther cultures of rice, Oryzae sativa L. Theor Appl Genet 70:113–116
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Núñez VM, Roca WM, Martínez CP (1989) El cultivo de anteras en el mejoramiento de arroz. Serie 04SR-07-02-CIAT, Cali, Colombia, 60p
Ou SH (1980) Pathogen variability and host resistance in rice blast disease. Ann Rev Phytopathol 18:167–187
Ou SH (1985) Rice diseases. Commonwealth Mycological Institute, Kew, Surrey, England, pp 109–201
Pauk J, Kertesz Z, Barabas Z (1988) Production of wheat lines from anther culture and their achievements in performance tests. Novenytermeless (Hungary) 37:197–203
Picard E, Parisot C, Blanchard P, Brabant P, Causse M, Doussinault G, Trotlet M, Rousset M (1988) Comparison of the doubled-haploid method with other breeding procedures in wheat (Triticum aestivum) when applied to populations. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genetic Symp. July 13–19, 1988. Inst Plant Sci Res Cambridge, UK, pp 1155–1159
Quimio CA, Zapata FJ (1990) Diallel analysis of callus induction and green-plant regeneration in rice anther culture. Crop Sci 30:188–192
Raina SK (1989) Tissue culture in rice improvement: Status and potential. Adv Agron 42:339–397
Rosero MJ (1979) Breeding for blast resistance at CIAT. In: Proc Rice Blast Workshop. Int Rice Res Inst, PO Box 930 Manila, Philippines, pp 63–67
Roumen EC (1992) Small differential interaction for partial resistance in rice cultivars to virulent isolates of the blast pathogen. Euphytica 64:143–148
Sanint LR, Martínez CP, Ramírez A, Lentini Z (1996) Rice breeding using anther culture or pedigree: a cost/benefit analysis. Crop Sci (in press)
Sarkarung S (1991) A simplified crossing method for rice breeding. SERIE ISBN 958-9183-33-6. CIAT. Cali, Colombia
SAS Institute, Inc (1990) User's guide, version 6, 4th edn Cary, North Carolina
Wang GL, Mackill DJ, Bonman MJ, McCouch SR, Champoux MC, Nelson RJ (1994) RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421–1434
Weeraratne H, Martínez CP, Jennings PR (1981) Genetic strategies in breeding for resistance to rice blast Pyricularia oryzae in Colombia. In: IRAT-GERDAT (ed) Proc Symp Rice Resistance to Blast, March 18–21, 1981. Montpellier, France, pp 305–311
Yu ZH, Mackill DJ, Bonman JM, Tanksley SD (1991) Tagging genes for blast resistance in rice via linkage to RFLP markers. Theor Appl Genet 81:471–476
Zeigler RS, Tohme J, Nelson RJ, Levy M, Correa Victoria FJ (1994) Lineage exclusion: A proposal to link blast population analysis to resistance breeding. In: Zeigler RS, Leong SA, Teng PS (eds) Rice blast disease. Commonwealth Agricultural Bureau International, Wallingford, UK, pp 267–292
Zeigler RS, Couc XL, Scott RP, Bernardo MA, Chen D, Valent B, Nelson RJ (1995) The relationship between lineage and virulence in P. grisea in the Philippines. Phytopathology 85:443–451
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Martínez, C.P., Victoria, F.C., Amézquita, M.C. et al. Comparison of rice lines derived through anther culture and the pedigree method in relation to blast (Pyricularia grisea Sacc.) resistance. Theoret. Appl. Genetics 92, 583–590 (1996). https://doi.org/10.1007/BF00224562
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224562