Summary
Genetic variants were found among over 6,000 primary plants (R1) regenerated from embryogenic tissue cultures of eight high tannin sorghums [Sorghum bicolor (L.) Moench]. Field assessment of somaclonal variation has progressed to the R2 population, with over 48,000 R2 seedlings (27,000 plants) in 1,126 rows from 1,055 R1 plants. A total of 43 variant phenotypes was recovered, including several types of chlorophyll deficiencies, dwarfism, short culm, sterility, narrow leaf, and several previously unreported variants, such as ragged leaf, multibranched heads, and Hydra, a developmental variant which produces large numbers of panicles. Variation production greatly depends on parent genotype and appears to increase with increasing time in cultures. The toal average somaclonal variation rate (based per 100 R1 plants) and somaclonal variant frequency (based per 100 R2 plants) estimated in the tested population were 11.3 and 1.6, respectively. Chimerism was found in regenerants. The estimated size of the mutated sector carried by mutant regenerants ranged from the whole plant to less than 3% of a single head. The average proportion of mutated R1 heads carrying large (80%–100%), medium (40%–80%), and small (<40%) mutated sectors was 38.7%, 26.0% and 35.3%, respectively. Some sector mutations do not appear until the R3 generation. In order to avoid losing variants, the population for selecting somaclonal variation should be as large as possible. Some of these variants found may be useful for further study or for use in breeding programs.
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References
Armstrong CL, Phillips RL (1988) Genetic and cytogenetic variation in plants regenerated from organogenic and friable embryogenic tissue cultures of maize. Crop Sci 28:363–369
Bhaskaran S, Smith RH, Schertz K (1983) Sodium chloride tolerant callus of Sorghum bicolor (L.) Moench. Z Pflanzenphysiol 112:459–463
Bhaskaran S, Smith RH, Paliwal S, Schertz K (1987) Somaclonal variation from Sorghum bicolor (L.) Moench cell culture. Plant Cell Tiss Org Cult 9:189–196
Brar DS, Rambold S, Gamborg O, Constabel F (1979) Tissue culture of corn and sorghum. Z Pflanzenphysiol 95:377–388
Butler LG (1989) Sorghum polyphenols. In: Cheeke PR (ed) Toxicants of plant origin, vol IV, Phenolics. CRC Press, Boca Raton/Fl, pp 95–121
Butler LG, Roger J, Mehansho H, Carlson D (1986) Dietary effects of tannin. In: Harborne J, Middleton E, Cody V (eds) Plant flavonoids in biology and medicine: biochemical pharmacological, and structure-activity relationships. Alan R. Liss, New York, pp 141–157
Cai T, Butler L (1990) Plant regeneration from embryogenic callus initiated from immature inflorescence of high tannin sorghums. Plant Cell Tiss Org Cult (in press)
Cai T, Daly B, Butler L (1987) Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghums. Plant Cell Tiss Org Cult 9:245–252
Cooper DB, Sears RG, Lookhart GL, Jones BL (1986) Heritable somaclonal variation in gliadin proteins of wheat plants derived from immature embryo callus culture. Theor Appl Genet 71:784–790
Duncan RR (1989) Sorghum breeding for stress tolerance: tissue culture and implication for Africa. In: Ketchum JLF, Gamborg OL (eds) The role of tissue culture and novel genetic technologies in crop improvement. Proc 3rd IPBNet Conf, 8–12 January, Nairobi. TCCP, Colorado State University, Fort Coffins, pp25–34
Evans DA, Sharp WR (1986) Applications of somaclonal variation. Bio/Technol 4:528–532
Fukui K (1983) Sequential occurence of mutations in a growing rice callus. Theor Appl Genet 65:225–230
Gaul H (1964) Mutations in plant breeding. Radiat Bot 4:155–232
Hanning GE, Kumar AS, Nabors MW (1989) In vitro selection in cereal and legume tissue culture and field evaluation of regenerated progeny. In: Ketchum JFL, Gamborg OL (eds) The role of tissue culture and novel genetic technologies in crop improvement. Proc 3rd IPBNet Conf, 8–12 January, Nairobi. TCCP, Colorado State University, Fort Coffins, pp 44–50
Hoffmann F, Wenzel G (1981) Self-compatibility in microsporederived doubled-haploid rye lines and single grain selection for alkylresorcinol content. Theor Appl Genet 60:129–133
Jordan MC, Larter EN (1985) Somaclonal variation in triticale (× Triticosecale Wittmack) CV Carman. Can J Genet Cytol 27:151–157
Karp A (1989) Can genetic instability be controlled in plant tissue cultures? Int Tiss Cult Newsl (IAPTC) 58:2–11
Karper RE, Conner AB (1931) Inheritance of chlorophyll characters in sorghum. Genetics 16:291–308
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214
Larkin PJ, Ryan SA, Brettel RIS, Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor Appl Genet 67:443–455
Lee M, Phillips RL (1987) Genetic variants in progeny of regenerated maize plants. Genome 29:834–838
Ma HT, Liang GH (1985) Studies on culture of immature sorghum embryos in vitro and variations of regenerated plants. Acta Genet Sin 12:350–357
Ma HT, Gu MH, Liang GH (1987) Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) Moench. Theor Appl Genet 73:389–394
Maddock SE, Semple JT (1986) Field assessment of somaclonal variation in wheat. J Exp Bot 37:1065–1078
Maddock SE, Risiott R, Parmar S, Jones MGK, Shewry PR (1985) Somaclonal variation in the gliadin patterns of grains of regenerated wheat plants. J Exp Bot 36:1976–1984
Oberthur EE, Nicholson RL, Butler LG (1983) Presence of polyphenolic materials, including condensed tannins, in sorghum callus. J Agric Food Chem 31:660–662
Pickering RA (1989) Plant regeneration and variants from calli derived from immature embryos of diploid barley (Hordeum vulgare L.) and H. vulgare L. × H. bulbosum L. crosses. Theor Appl Genet 78:105–112
Poethig RS (1988) Heterochronic mutation affecting shoot development in maize. Genetics 119:959–973
Price ML, Van Scoyoc S, Butler LG (1978) A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 26:1214–1218
Ramulu KS (1975) Mutation breeding in sorghum. Z Pflanzenzuecht 74:1–17
Ryan SA, Larkin PJ, Ellison FW (1987) Somaclonal variation in some agronomic and quality characters in wheat. Theor Appl Genetl 74:77–82
Smith RH, Bhaskaran S, Schertz K (1983) Sorghum plant regeneration from aluminum selection media. Plant Cell Rep 2:129–132
Smith RH, Bhaskaran S, Miller FR (1985) Screening for drought tolerance in sorghum using cell culture. In Vitro Cell Der Biol 21:541–545
Sun ZX, Zhao CZ, Zheng KL, Qi XF, Fu YP (1983) Somaclonal genetics of rice, Oryza sativa L. Theor Appl Genet 67:67–73
Tissue Culture for Crop Project (TCCP) Progress Report (1987) Ketchum JFL, Gamborg OL, Hanning GE, Nabors MW (eds) Shorgum TCCP, Colorado State University, Fort Collins, pp 33–37
Zehr BE, Williams ME, Duncan DR, Widholm JM (1987) Somaclonal variation in the progeny of plants regenerated from callus cultures of seven inbred lines of maize. Can J Bot 65:491–499
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Communicated by A.R. Hallauer
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Cai, T., Ejeta, G., Axtell, J.D. et al. Somaclonal variation in high tannin sorghums. Theoret. Appl. Genetics 79, 737–747 (1990). https://doi.org/10.1007/BF00224238
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DOI: https://doi.org/10.1007/BF00224238