Abstract
The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored the direct amplification of minisatellite DNA by PCR (DAMD-PCR) as a means for generating DNA probes that are useful for detecting DNA polymorphisms and DNA fingerprinting in wheat. This technique was facilitated by high-stringency PCR with known plant and animal minisatellite core sequences as primers on wheat genomic DNA. The products of DAMD-PCR from Triticum aestivum, T. durum, T. monococcum, T. speltoides and T. tauschii showed a high degree of polymorphism and the various genomes could be identified. Cloning of the DAMD-PCR products and subsequent Southern hybridization frequently revealed polymorphic probes showing a good degree of genome specificity. In addition, polymorphic, single locus, and moderately dispersed PCR products were cloned that may have a potential for DNA fingerprinting. Our experiments were limited primarily to diploid wheats and the results indicated that DAMD-PCR may isolate genome-specific probes from wild diploid wheat species that could be used to monitor genome introgression into hexaploid wheat.
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Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Bjourson AJ, Cooper JE (1992) Band-stab PCR: a simple technique or the purification of individual PCR products. Nucleic Acids Res 20:4675
Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction fragment length polymorphisms to high specific activity. Anal Biochem 132:6–13
Heath DD, Iwama GK, Devlin RH (1993) PCR primed with VNTR core sequences yields species specific patterns and hypervariable probes. Nucleic Acids Res 21:5782–5785
Jeffreys AJ, Wilson V, Thein SL (1985 a) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73
Jeffreys AJ, Wilson V, Thein SL (1985 b) Individual-specific ‘fingerprints’ of human DNA. Nature 316:76–79
McIntyre CL, Periera S, Mason LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640
Murray MJ, Haldeman BA, Grant FJ, O'Hara N (1988) Probing the human genome with minisatellite-like sequences from the human coagulation factor-VII gene. Nucleic Acids Res 16:4166
Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235:1616–1622
Rayburn AL, Gill BS (1987) Isolation of a D genome-specific repeated DNA sequence from Agilops sqarrosa. Plant Mol Biol Rep 4:102–109
Roder MS, Plaschke J, Konig SU, Borner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333
Saghai-Maroof MA, Solinam KM, Jorgensen RA, Allard, RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Vassart G, Georges M, Monsieur R, Brocas H, Lequarre A-S, Christophe D (1987) A sequence of M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235:683–684
Vergnaud G (1989) Polymers of random short oligonucleotides detect polymorphic loci in the human genome. Nucleic Acids Res 17:7623–7630
Wang K, Koop BF, Hood L (1994) A simple method using T4 DNA polymerase to clone polymerase chain reaction products. Biotechniques 17:236–238
Winberg BC, Zhou Z, Dallas JF, McIntyre CL, Gustafson JP (1993) Characterization of minisatellite sequences from Oryza sativa. Genome 36:978–983
Zhou Z, Gustafson JP (1995) Genetic variation detected by DNA fingerprinting with a rice minisatellite probe in Oryza sativa L. Theor Appl Genet 91:481–488
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Communicated by G. E. Hart
This paper reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by the USDA or the University of Missouri. Contribution from the University of Missouri, the Agricultural Experimental Station and U.S. Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, journal series No. 12523
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Somers, D.J., Zhou, Z., Bebeli, P.J. et al. Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell) amplified with minisatellite core sequences. Theoret. Appl. Genetics 93, 982–989 (1996). https://doi.org/10.1007/BF00224102
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DOI: https://doi.org/10.1007/BF00224102